FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2049079521> ?p ?o ?g. }

• W2049079521 endingPage "96" @default.
• W2049079521 startingPage "90" @default.
• W2049079521 abstract "Immunohistochemical staining is important for diagnosis and therapeutic decision making but the results may vary when different detection systems are used. To analyze this, 5 different labeled polymer immunohistochemical detection systems, REAL EnVision, EnVision Flex, EnVision Flex+ (Dako, Glostrup, Denmark), NovoLink (Novocastra Laboratories Ltd, Newcastle Upon Tyne, UK) and UltraVision ONE (Thermo Fisher Scientific, Fremont, CA) were tested using 12 different, widely used mouse and rabbit primary antibodies, detecting nuclear, cytoplasmic, and membrane antigens. Serial sections of multitissue blocks containing 4% formaldehyde fixed paraffin embedded material were selected for their weak, moderate, and strong staining for each antibody. Specificity and sensitivity were evaluated by subjective scoring and digital image analysis. At optimal primary antibody dilution, digital image analysis showed that EnVision Flex+ was the most sensitive system (P<0.005), with means of 8.3, 13.4, 20.2, and 41.8 gray scale values stronger staining than REAL EnVision, EnVision Flex, NovoLink, and UltraVision ONE, respectively. NovoLink was the second most sensitive system for mouse antibodies, but showed low sensitivity for rabbit antibodies. Due to low sensitivity, 2 cases with UltraVision ONE and 1 case with NovoLink stained false negatively. None of the detection systems showed any distinct false positivity, but UltraVision ONE and NovoLink consistently showed weak background staining both in negative controls and at optimal primary antibody dilution. We conclude that there are significant differences in sensitivity, specificity, costs, and total assay time in the immunohistochemical detection systems currently in use." @default.
• W2049079521 created "2016-06-24" @default.
• W2049079521 creator A5015030195 @default.
• W2049079521 creator A5029279187 @default.
• W2049079521 creator A5036000503 @default.
• W2049079521 creator A5042264603 @default.
• W2049079521 creator A5055306958 @default.
• W2049079521 creator A5067025453 @default.
• W2049079521 creator A5082443222 @default.
• W2049079521 date "2010-01-01" @default.
• W2049079521 modified "2023-09-27" @default.
• W2049079521 title "Evaluation of 5 Different Labeled Polymer Immunohistochemical Detection Systems" @default.
• W2049079521 cites W1973214450 @default.
• W2049079521 cites W2005207656 @default.
• W2049079521 cites W2008691306 @default.
• W2049079521 cites W2020632674 @default.
• W2049079521 cites W2048708488 @default.
• W2049079521 cites W2087033262 @default.
• W2049079521 cites W2090102058 @default.
• W2049079521 cites W2099566069 @default.
• W2049079521 cites W2111106093 @default.
• W2049079521 cites W2159900400 @default.
• W2049079521 cites W2170950989 @default.
• W2049079521 cites W4238951368 @default.
• W2049079521 cites W4246256927 @default.
• W2049079521 cites W4255045875 @default.
• W2049079521 doi "https://doi.org/10.1097/pai.0b013e3181b0eaad" @default.
• W2049079521 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19661787" @default.
• W2049079521 hasPublicationYear "2010" @default.
• W2049079521 type Work @default.
• W2049079521 sameAs 2049079521 @default.
• W2049079521 citedByCount "26" @default.
• W2049079521 countsByYear W20490795212012 @default.
• W2049079521 countsByYear W20490795212013 @default.
• W2049079521 countsByYear W20490795212015 @default.
• W2049079521 countsByYear W20490795212016 @default.
• W2049079521 countsByYear W20490795212017 @default.
• W2049079521 countsByYear W20490795212018 @default.
• W2049079521 countsByYear W20490795212019 @default.
• W2049079521 countsByYear W20490795212020 @default.
• W2049079521 countsByYear W20490795212021 @default.
• W2049079521 countsByYear W20490795212022 @default.
• W2049079521 crossrefType "journal-article" @default.
• W2049079521 hasAuthorship W2049079521A5015030195 @default.
• W2049079521 hasAuthorship W2049079521A5029279187 @default.
• W2049079521 hasAuthorship W2049079521A5036000503 @default.
• W2049079521 hasAuthorship W2049079521A5042264603 @default.
• W2049079521 hasAuthorship W2049079521A5055306958 @default.
• W2049079521 hasAuthorship W2049079521A5067025453 @default.
• W2049079521 hasAuthorship W2049079521A5082443222 @default.
• W2049079521 hasConcept C125473707 @default.
• W2049079521 hasConcept C142724271 @default.
• W2049079521 hasConcept C147483822 @default.
• W2049079521 hasConcept C153911025 @default.
• W2049079521 hasConcept C159654299 @default.
• W2049079521 hasConcept C184112429 @default.
• W2049079521 hasConcept C185592680 @default.
• W2049079521 hasConcept C203014093 @default.
• W2049079521 hasConcept C204232928 @default.
• W2049079521 hasConcept C204787440 @default.
• W2049079521 hasConcept C21269302 @default.
• W2049079521 hasConcept C2779484032 @default.
• W2049079521 hasConcept C2909954168 @default.
• W2049079521 hasConcept C31972630 @default.
• W2049079521 hasConcept C41008148 @default.
• W2049079521 hasConcept C71924100 @default.
• W2049079521 hasConcept C74864618 @default.
• W2049079521 hasConcept C86803240 @default.
• W2049079521 hasConceptScore W2049079521C125473707 @default.
• W2049079521 hasConceptScore W2049079521C142724271 @default.
• W2049079521 hasConceptScore W2049079521C147483822 @default.
• W2049079521 hasConceptScore W2049079521C153911025 @default.
• W2049079521 hasConceptScore W2049079521C159654299 @default.
• W2049079521 hasConceptScore W2049079521C184112429 @default.
• W2049079521 hasConceptScore W2049079521C185592680 @default.
• W2049079521 hasConceptScore W2049079521C203014093 @default.
• W2049079521 hasConceptScore W2049079521C204232928 @default.
• W2049079521 hasConceptScore W2049079521C204787440 @default.
• W2049079521 hasConceptScore W2049079521C21269302 @default.
• W2049079521 hasConceptScore W2049079521C2779484032 @default.
• W2049079521 hasConceptScore W2049079521C2909954168 @default.
• W2049079521 hasConceptScore W2049079521C31972630 @default.
• W2049079521 hasConceptScore W2049079521C41008148 @default.
• W2049079521 hasConceptScore W2049079521C71924100 @default.
• W2049079521 hasConceptScore W2049079521C74864618 @default.
• W2049079521 hasConceptScore W2049079521C86803240 @default.
• W2049079521 hasIssue "1" @default.
• W2049079521 hasLocation W20490795211 @default.
• W2049079521 hasLocation W20490795212 @default.
• W2049079521 hasOpenAccess W2049079521 @default.
• W2049079521 hasPrimaryLocation W20490795211 @default.
• W2049079521 hasRelatedWork W157369640 @default.
• W2049079521 hasRelatedWork W1971407250 @default.
• W2049079521 hasRelatedWork W1992478450 @default.
• W2049079521 hasRelatedWork W1997541390 @default.
• W2049079521 hasRelatedWork W2060937867 @default.
• W2049079521 hasRelatedWork W2067097521 @default.
• W2049079521 hasRelatedWork W2074123101 @default.

• next