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• W2049104046 abstract "α-Neup5Ac-(2→6)-d-GalpNAc, the carbohydrate portion of sialyl–Tn epitope of the tumor-associated carbohydrate antigen, was prepared by a whole-cell reaction through the combination of recombinant Escherichia coli strains and Corynebacterium ammoniagenes. Two recombinant E. coli strains overexpressed the CMP-Neup5Ac biosynthetic genes and the α-(2→6)-sialyltransferase gene of Photobacterium damsela. C. ammoniagenes contributed to the production of UTP from orotic acid. α-Neup5Ac-(2→6)-d-GalpNAc was accumulated at 87 mM (45 g/L) after a 25-h reaction starting from orotic acid, N-acetylneuraminic acid, and 2-acetamide-2-deoxy-d-galactose." @default.
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• W2049104046 date "2001-02-01" @default.
• W2049104046 modified "2023-10-10" @default.
• W2049104046 title "Large-scale production of the carbohydrate portion of the sialyl–Tn epitope, α-Neup5Ac-(2→6)-d-GalpNAc, through bacterial coupling" @default.
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• W2049104046 doi "https://doi.org/10.1016/s0008-6215(01)00007-6" @default.
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