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- W2049113838 abstract "Baseline separation of the isomers of retinol using reversed-phase high-performance liquid chromatography (HPLC) in less than 30 min is presented. A new approach to the detection of retinol using electrochemical detection is developed. The oxidative electrochemistry of retinol is studied at a glassy-carbon electrode using coulometry, ultraviolet-visible spectrophotometry and HPLC. Amperometric detection in HPLC for retinol provided a linear response from 0 to 1.5 micrograms/ml and a detection limit of 4.1 ng/ml. Electrochemical detection was compared to ultraviolet-visible absorbance detection for the determination of retinol in human serum extracts. Good agreement is found for the results obtained with the two detectors." @default.
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- W2049113838 title "Reversed-phase high-performance liquid chromatographic separation and electrochemical detection of retinol and its isomers" @default.
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- W2049113838 doi "https://doi.org/10.1016/0378-4347(87)80092-0" @default.
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