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- W2049161775 abstract "Fluorophores in close proximity to metallic nanoparticles (NPs) are promising systems to develop sensors, especially in the fields of cellular tracking and imaging. Metallic NPs can modify the emission of fluorescent molecules by increasing the excitation and emission rates (plasmonic enhancement). Using the orbital tracking method, here we track 20nm gold NPs inside the nuclei of Chok1 cells that have been transfected with a plasmid expressing H2B-mCherry or H2B-GFP histone proteins. Then the gold nanoparticles are inserted inside the nuclei. We show that, during tracking, gold NPs have different movement inside the nucleus. We observed a confined movement in which the nanoparticles are restricted in specific areas and long directed movement connecting these confined movement areas. During the movement the gold nanoparticles interact with the surroundings and give fluorescent enhancement of specific fluorophores with different emission spectra. The enhancement due to the proximity of gold NP and fluorophores inside the nucleus appears as emission spikes of different colors presumably according to the color of the fluorescent molecules in close proximity at the nanoparticles. In this study to discriminate between the different emission colors observed along the nanoparticle trajectories, we use the general polarization function (GP). The GP value changes suddenly along the trajectory and along the orbit we use during tracking and gave us information about the color emission of the proteins that are in close proximity at the gold nanoparticles at the nanoscale. Work supported in part by NIH grants P50 GM076516 and P41 GM103540." @default.
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- W2049161775 date "2014-01-01" @default.
- W2049161775 modified "2023-09-26" @default.
- W2049161775 title "Nanoimaging of the Cell Nucleus Using Gold Nanoparticles" @default.
- W2049161775 doi "https://doi.org/10.1016/j.bpj.2013.11.452" @default.
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