FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2049166379> ?p ?o ?g. }

• W2049166379 abstract "Sequencing of cDNA clones has shown that the carboxy terminal domain of the core protein of large proteoglycans (aggrecans) from human cartilage contains an epidermal growth factor-like (EGF-like) domain which is alternatively spliced. In a previous study it was found that the domain of the translated protein can be recognized by polyclonal antibodies to mouse EGF. A competitive enzyme-linked immunoabsorbent (ELISA) assay has been developed to evaluate the EGF-like domain content of aggrecans at various ages and in osteoarthritis. Fetal aggrecans digested with protease free chondroitinase ABC were adsorbed on polyvinyl chloride microtiter plates followed by blocking with bovine serum albumin and goat serum. Mixtures of known amounts of protein of digested aggrecans and constant amounts of anti-mouse EGF antibodies were incubated and added to plates. The second antibody was peroxidase-conjugate F(ab')2. Fetal, newborn and child aggrecan proteins have a higher content of EGF-like domain than aggrecan proteins from cartilage of older humans. Three areas of cartilages from osteoarthritic joints were separated: cartilages with normal macroscopic appearance, erosion border cartilage and osteophytic cartilage. Values derived from these samples were compared with values derived from nonosteoarthritic aged humans. The content of aggrecans from osteoarthritic cartilage with normal macroscopic appearance was similar to or slightly lower than the latter. The aggrecans from osteophytes had a higher EGF-like domain content. The aggrecans from the erosion border had a variable content, close to noneroded cartilages, to osteophytes or in between the values obtained for noneroded cartilages and for osteophytes. Variations in the amount of newly synthesized aggrecans, in the proteolysis of the carboxy terminal domain of aggrecans and in the alternating splicing of the EGF-like domain might explain the results shown here." @default.
• W2049166379 created "2016-06-24" @default.
• W2049166379 creator A5016773598 @default.
• W2049166379 creator A5056583953 @default.
• W2049166379 creator A5080452716 @default.
• W2049166379 date "1993-07-01" @default.
• W2049166379 modified "2023-10-17" @default.
• W2049166379 title "The epidermal growth factor-like domain of the large proteoglycans from articular cartilage (aggrecans). Estimate of content at different ages and in osteoarthritis" @default.
• W2049166379 cites W126032055 @default.
• W2049166379 cites W1431687529 @default.
• W2049166379 cites W1489673976 @default.
• W2049166379 cites W1521949639 @default.
• W2049166379 cites W1542080278 @default.
• W2049166379 cites W1564232667 @default.
• W2049166379 cites W1596577347 @default.
• W2049166379 cites W1605014062 @default.
• W2049166379 cites W1775749144 @default.
• W2049166379 cites W1913744980 @default.
• W2049166379 cites W1987847135 @default.
• W2049166379 cites W2002974844 @default.
• W2049166379 cites W2006245225 @default.
• W2049166379 cites W2037020755 @default.
• W2049166379 cites W2054703329 @default.
• W2049166379 cites W2066375220 @default.
• W2049166379 cites W2073796792 @default.
• W2049166379 cites W2101981774 @default.
• W2049166379 cites W2136868816 @default.
• W2049166379 cites W2158020454 @default.
• W2049166379 cites W2231795975 @default.
• W2049166379 cites W2313835802 @default.
• W2049166379 cites W2324969123 @default.
• W2049166379 cites W2341720846 @default.
• W2049166379 cites W435729752 @default.
• W2049166379 cites W576556375 @default.
• W2049166379 doi "https://doi.org/10.1016/s1063-4584(05)80089-5" @default.
• W2049166379 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15449424" @default.
• W2049166379 hasPublicationYear "1993" @default.
• W2049166379 type Work @default.
• W2049166379 sameAs 2049166379 @default.
• W2049166379 citedByCount "2" @default.
• W2049166379 countsByYear W20491663792015 @default.
• W2049166379 crossrefType "journal-article" @default.
• W2049166379 hasAuthorship W2049166379A5016773598 @default.
• W2049166379 hasAuthorship W2049166379A5056583953 @default.
• W2049166379 hasAuthorship W2049166379A5080452716 @default.
• W2049166379 hasConcept C105702510 @default.
• W2049166379 hasConcept C142724271 @default.
• W2049166379 hasConcept C153911025 @default.
• W2049166379 hasConcept C170493617 @default.
• W2049166379 hasConcept C185592680 @default.
• W2049166379 hasConcept C197534660 @default.
• W2049166379 hasConcept C204787440 @default.
• W2049166379 hasConcept C2776164576 @default.
• W2049166379 hasConcept C2776362946 @default.
• W2049166379 hasConcept C2780550940 @default.
• W2049166379 hasConcept C3020332539 @default.
• W2049166379 hasConcept C55493867 @default.
• W2049166379 hasConcept C71924100 @default.
• W2049166379 hasConcept C86803240 @default.
• W2049166379 hasConceptScore W2049166379C105702510 @default.
• W2049166379 hasConceptScore W2049166379C142724271 @default.
• W2049166379 hasConceptScore W2049166379C153911025 @default.
• W2049166379 hasConceptScore W2049166379C170493617 @default.
• W2049166379 hasConceptScore W2049166379C185592680 @default.
• W2049166379 hasConceptScore W2049166379C197534660 @default.
• W2049166379 hasConceptScore W2049166379C204787440 @default.
• W2049166379 hasConceptScore W2049166379C2776164576 @default.
• W2049166379 hasConceptScore W2049166379C2776362946 @default.
• W2049166379 hasConceptScore W2049166379C2780550940 @default.
• W2049166379 hasConceptScore W2049166379C3020332539 @default.
• W2049166379 hasConceptScore W2049166379C55493867 @default.
• W2049166379 hasConceptScore W2049166379C71924100 @default.
• W2049166379 hasConceptScore W2049166379C86803240 @default.
• W2049166379 hasLocation W20491663791 @default.
• W2049166379 hasLocation W20491663792 @default.
• W2049166379 hasOpenAccess W2049166379 @default.
• W2049166379 hasPrimaryLocation W20491663791 @default.
• W2049166379 hasRelatedWork W1777800286 @default.
• W2049166379 hasRelatedWork W1795076303 @default.
• W2049166379 hasRelatedWork W1964442086 @default.
• W2049166379 hasRelatedWork W1980573958 @default.
• W2049166379 hasRelatedWork W2000257110 @default.
• W2049166379 hasRelatedWork W2003875220 @default.
• W2049166379 hasRelatedWork W2005227460 @default.
• W2049166379 hasRelatedWork W2007122444 @default.
• W2049166379 hasRelatedWork W2020114376 @default.
• W2049166379 hasRelatedWork W2041865050 @default.
• W2049166379 hasRelatedWork W2048664475 @default.
• W2049166379 hasRelatedWork W2057297291 @default.
• W2049166379 hasRelatedWork W2065497238 @default.
• W2049166379 hasRelatedWork W2078370061 @default.
• W2049166379 hasRelatedWork W2087813282 @default.
• W2049166379 hasRelatedWork W2143865964 @default.
• W2049166379 hasRelatedWork W2256063435 @default.
• W2049166379 hasRelatedWork W2260438337 @default.
• W2049166379 hasRelatedWork W2342423404 @default.
• W2049166379 hasRelatedWork W2411425406 @default.
• W2049166379 isParatext "false" @default.
• W2049166379 isRetracted "false" @default.
• W2049166379 magId "2049166379" @default.

• next