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- W2049203343 abstract "Abstract 2,4-Dinitro-5-fluoroaniline was reacted with various amino acids and proteins. The N -2,4-dinitro-5-aminophenyl (DNAP) derivatives of twenty amino acids were prepared and three solvent systems which can effect their separation on thin-layer chromatograms were developed. DNAP insulin was prepared and it was shown that N -terminal amino acids can be determined starting with 50 μg of this derivative or 100 μg of insulin. The color of DNAP amino acids can be intensified on thin-layer chromatograms by first exposing the chromatograms to nitrogen dioxide fumes and then spraying with a solution of β-naphthol and triethylamine in benzene; this permits the detection of as little as 0.1 μg (about one nanomole) of DNAP amino acid." @default.
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- W2049203343 date "1967-05-01" @default.
- W2049203343 modified "2023-10-16" @default.
- W2049203343 title "The use of 2,4-dinitro-5-fluoroaniline in the determination of protein structure on a micro scale" @default.
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- W2049203343 doi "https://doi.org/10.1016/0003-2697(67)90172-8" @default.
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