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- W2049204624 abstract "A hybrid plasmid containing a fragment of the Corynebacterium melassecola chromosome cloned into pBR325 restored growth of glutamate auxotrophs of Escherichia coli strains that have mutations in the genes for glutamate dehydrogenase and glutamate synthase. A 3.1-kilobase pair region was shown by complementation analysis and enzyme measurements to carry the glutamate dehydrogenase gene, gdh. Glutamate dehydrogenase encoded by gdh carried on recombinant plasmids was elevated over 100-fold in E. coli cells. The gdh promoter was located by in vitro fusion to a promoter-deficient galK gene." @default.
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- W2049204624 date "1990-01-01" @default.
- W2049204624 modified "2023-10-18" @default.
- W2049204624 title "Cloning and expression in Escherichia coli of the glutamate dehydrogenase gene, gdh, from Corynebacterium melassecola" @default.
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- W2049204624 doi "https://doi.org/10.1016/0922-338x(90)90236-p" @default.
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