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- W2049222500 abstract "The cDNA that encodes the human μ opioid receptor (hMOR) has been cloned and expressed in Spodoptera frugiperda (Sf9) cells using a nonlytic vector system. The coding sequence fused to the cleavable glycoprotein signal peptide gp 64, and a C-terminal histidine tag was placed under the transcriptional control of the Orgyia pseudotsugata multicapsid nucleopolyhedrosis virus immediate-early 2 (OpIE2) promoter. Transfected cells were selected using Zeocin resistance and the receptor was constitutively expressed at approximately 12 000 receptors per cell. Immunofluorescence images illustrated that more than 75% of the Sf9 cells expressed hMOR at the plasma membrane. This is the first report of the constitutive and heterologous expression of a G protein-coupled receptor in a stably transfected Sf9 cell line, under the control of the OpIE2 promoter." @default.
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- W2049222500 date "2002-05-01" @default.
- W2049222500 modified "2023-10-16" @default.
- W2049222500 title "Expression of the human μ opioid receptor in a stable Sf9 cell line" @default.
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- W2049222500 doi "https://doi.org/10.1016/s0168-1656(02)00008-1" @default.
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