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- W2049227426 abstract "A cell-free system for preparative gene expression is described. It is composed of DNA-free Escherichia coli extract and added plasmid DNA; coupled transcription-translation proceeds with a continuous flow of the feeding solution containing nucleoside triphosphates and amino acids. The system works at a high constant rate for tens of hours. The yield of synthesised proteins after 20-50 h is hundreds of micrograms from 1 ml of the reaction mixture. Electrophoretic analysis of translation products confirms synthesis of proteins of the expected molecular mass." @default.
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- W2049227426 date "1989-12-01" @default.
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- W2049227426 title "Gene expression in a cell-free system on the preparative scale" @default.
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- W2049227426 doi "https://doi.org/10.1016/0378-1119(89)90521-0" @default.
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