FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2049252219> ?p ?o ?g. }

• W2049252219 endingPage "32259" @default.
• W2049252219 startingPage "32254" @default.
• W2049252219 abstract "Elongation factor P (EFP) is a protein that stimulates the peptidyltransferase activity of fully assembled 70 S prokaryotic ribosomes and enhances the synthesis of certain dipeptides initiated by N-formylmethionine. This reaction appears conserved throughout species and is promoted in eukaryotic cells by a homologous protein, eIF5A. Here we ask whether the Escherichia coli gene encoding EFP is essential for cell viability. A kanamycin resistance (Kan R) gene was inserted near the N-terminal end of the efp gene and was cloned into a plasmid, pMAK705, that has a temperature-sensitive origin of replication. After transformation into a recA+ E. coli strain, temperature-sensitive mutants were isolated, and their chromosomal DNA was sequenced. Mutants containing the efp-Kan R gene in the chromosome grew at 33 °C only in the presence of the wild-type copy of the efp gene in the pMAK705 plasmid and were unable to grow at 44 °C. Incorporation of various isotopes in vivo suggests that translation is impaired in the efp mutant at 44 °C. At 44 °C, mutant cells are severely defective in peptide-bond formation. We conclude that the efp gene is essential for cell viability and is required for protein synthesis." @default.
• W2049252219 created "2016-06-24" @default.
• W2049252219 creator A5029523917 @default.
• W2049252219 creator A5066404557 @default.
• W2049252219 creator A5074420539 @default.
• W2049252219 creator A5090930538 @default.
• W2049252219 date "1997-12-01" @default.
• W2049252219 modified "2023-10-13" @default.
• W2049252219 title "The Gene Encoding the Elongation Factor P Protein Is Essential for Viability and Is Required for Protein Synthesis" @default.
• W2049252219 cites W1492229616 @default.
• W2049252219 cites W1527220194 @default.
• W2049252219 cites W1544068656 @default.
• W2049252219 cites W1569873859 @default.
• W2049252219 cites W164133302 @default.
• W2049252219 cites W1849300906 @default.
• W2049252219 cites W1868594534 @default.
• W2049252219 cites W1903945848 @default.
• W2049252219 cites W1910590028 @default.
• W2049252219 cites W1967465227 @default.
• W2049252219 cites W1980032360 @default.
• W2049252219 cites W1989794771 @default.
• W2049252219 cites W1993124128 @default.
• W2049252219 cites W1994954153 @default.
• W2049252219 cites W2019410656 @default.
• W2049252219 cites W2023280070 @default.
• W2049252219 cites W2030985480 @default.
• W2049252219 cites W2032467543 @default.
• W2049252219 cites W2041415001 @default.
• W2049252219 cites W204476414 @default.
• W2049252219 cites W2053760358 @default.
• W2049252219 cites W2060985277 @default.
• W2049252219 cites W2064187335 @default.
• W2049252219 cites W2068444787 @default.
• W2049252219 cites W2070053383 @default.
• W2049252219 cites W2070434234 @default.
• W2049252219 cites W2080195917 @default.
• W2049252219 cites W2088484900 @default.
• W2049252219 cites W2138270253 @default.
• W2049252219 cites W2142583547 @default.
• W2049252219 cites W2150832645 @default.
• W2049252219 cites W2155373804 @default.
• W2049252219 doi "https://doi.org/10.1074/jbc.272.51.32254" @default.
• W2049252219 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9405429" @default.
• W2049252219 hasPublicationYear "1997" @default.
• W2049252219 type Work @default.
• W2049252219 sameAs 2049252219 @default.
• W2049252219 citedByCount "80" @default.
• W2049252219 countsByYear W20492522192012 @default.
• W2049252219 countsByYear W20492522192013 @default.
• W2049252219 countsByYear W20492522192014 @default.
• W2049252219 countsByYear W20492522192016 @default.
• W2049252219 countsByYear W20492522192017 @default.
• W2049252219 countsByYear W20492522192018 @default.
• W2049252219 countsByYear W20492522192019 @default.
• W2049252219 countsByYear W20492522192020 @default.
• W2049252219 countsByYear W20492522192022 @default.
• W2049252219 countsByYear W20492522192023 @default.
• W2049252219 crossrefType "journal-article" @default.
• W2049252219 hasAuthorship W2049252219A5029523917 @default.
• W2049252219 hasAuthorship W2049252219A5066404557 @default.
• W2049252219 hasAuthorship W2049252219A5074420539 @default.
• W2049252219 hasAuthorship W2049252219A5090930538 @default.
• W2049252219 hasBestOaLocation W20492522191 @default.
• W2049252219 hasConcept C104317684 @default.
• W2049252219 hasConcept C105580179 @default.
• W2049252219 hasConcept C112950240 @default.
• W2049252219 hasConcept C125411270 @default.
• W2049252219 hasConcept C139124968 @default.
• W2049252219 hasConcept C149364088 @default.
• W2049252219 hasConcept C169760540 @default.
• W2049252219 hasConcept C185592680 @default.
• W2049252219 hasConcept C191897082 @default.
• W2049252219 hasConcept C192562407 @default.
• W2049252219 hasConcept C33947775 @default.
• W2049252219 hasConcept C3675279 @default.
• W2049252219 hasConcept C54355233 @default.
• W2049252219 hasConcept C55493867 @default.
• W2049252219 hasConcept C67705224 @default.
• W2049252219 hasConcept C70721500 @default.
• W2049252219 hasConcept C75442077 @default.
• W2049252219 hasConcept C86803240 @default.
• W2049252219 hasConcept C88478588 @default.
• W2049252219 hasConcept C95444343 @default.
• W2049252219 hasConceptScore W2049252219C104317684 @default.
• W2049252219 hasConceptScore W2049252219C105580179 @default.
• W2049252219 hasConceptScore W2049252219C112950240 @default.
• W2049252219 hasConceptScore W2049252219C125411270 @default.
• W2049252219 hasConceptScore W2049252219C139124968 @default.
• W2049252219 hasConceptScore W2049252219C149364088 @default.
• W2049252219 hasConceptScore W2049252219C169760540 @default.
• W2049252219 hasConceptScore W2049252219C185592680 @default.
• W2049252219 hasConceptScore W2049252219C191897082 @default.
• W2049252219 hasConceptScore W2049252219C192562407 @default.
• W2049252219 hasConceptScore W2049252219C33947775 @default.
• W2049252219 hasConceptScore W2049252219C3675279 @default.
• W2049252219 hasConceptScore W2049252219C54355233 @default.
• W2049252219 hasConceptScore W2049252219C55493867 @default.
• W2049252219 hasConceptScore W2049252219C67705224 @default.

• next