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• W2049319023 abstract "The present study demonstrates that mitochondrial cytochrome c reacts with the thiol-reacting agent N-ethylmaleimide (NEM) to produce a one NEM-adducted cytochrome c. Mitochondrial cytochrome c also reacts with 5,5'-dithio-bis-(2-nitrobenzoic acid) and 1-chloro-2,4-dinitrobenzene in a manner prevented with NEM or iodoacetic acid (IAA). NEM-treated cytochrome c has lower reducibility and lower function to support mitochondrial oxygen consumption. These findings suggest that mitochondrial cytochrome c contains a reactive thiol that is involved in the functions of cytochrome c for mitochondria. Nitric oxide reacts with the cytochrome c thiol to generate S-nitroso (SNO)-cytochrome c in a manner prevented with NEM or IAA. SNO-cytochrome c has lower reducibility and function to support mitochondrial oxygen consumption, similar to NEM-treated cytochrome c." @default.
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• W2049319023 date "2006-04-01" @default.
• W2049319023 modified "2023-09-25" @default.
• W2049319023 title "Mitochondrial cytochrome c reacts with nitric oxide via S-nitrosation" @default.
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• W2049319023 doi "https://doi.org/10.1016/j.bbrc.2006.02.067" @default.
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