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• W2049353829 abstract "Abstract 1. 1. Desaturase activity of microsomal preparations from sheep liver was low compared with that of similar preparations from rat and chicken liver; highest activity was found in microsomes from chicken liver. Desaturase activity of microsomes from perinephric adipose tissue relative to that of microsomes from liver was markedly higher in sheep compared with rat and chicken; lowest activity was found in the chicken. 2. 2. Microsomal desaturase activity of the jejunal mucosa, liver and perinephric and subcutaneous adipose tissue of the sheep increased in that order. 3. 3. Desaturase activity of microsomes from lactating mammary tissue of the sheep was of the same order as that found in microsomes from perinephric and subcutaneous adipose tissue. Weaning the lambs resulted in decreased desaturase activity in microsomes from the mammary tissue of the respective ewes. 4. 4. Microsomes from sheep liver, in the presence of l -glycerol 3-phosphate and particle-free supernatant, incorporated [1-14C]-stearate mainly into phospholipids. Similar preparations from the chicken incorporated [1-14C]-stearate mainly into triacylglycerol, whereas in preparations from the rat significant incorporation into phospholipid and triacylglycerol occurred. 5. 5. Liver slices from lactating ewes incorporated [1-14C]-acetate mainly into phospholipids, whereas incorporation by slices of lactating mammary tissue was largely into triacylglycerols. Incorporation of [1-14C]-stearate into slices of liver and mamary tissue was low, but followed a similar pattern to that found for acetate incorporation. 6. 6. A higher ratio of mono-unsaturated to saturated fatty acids was observed when tissue preprations were incubated with [1-14C]-acetate compared with [1-14C]-stearate. 7. 7. The possibility is discussed that a preferential desaturation of endogenously synthesized fatty acids takes place." @default.
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• W2049353829 date "1974-05-01" @default.
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• W2049353829 title "Desaturation of long-chain fatty acids by tissue preparations of the sheep, rat and chicken" @default.
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• W2049353829 doi "https://doi.org/10.1016/0305-0491(74)90045-5" @default.
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