Matches in SemOpenAlex for { <https://semopenalex.org/work/W2049389638> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2049389638 endingPage "130" @default.
- W2049389638 startingPage "122" @default.
- W2049389638 abstract "The differentiating and antitumor activities of 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3) in vitro and 1α-hydroxyvitamin D3 (1α(OH)D3) in vivo were studied with a human osteosarcoma cell line (OST strain). Antitumor activity was estimated with the use of 3-(4,5-dimethylthiazol)-2, 5-diphenyltetrazolium bromide (MTT) assay, colony-forming assay, and athymic mouse assay. The intracellular alkaline phosphatase (ALP) activity of tumor cells and production of bone Gla protein (BGP) in culture media were measured to mark osteoblastic differentiation. In addition, the combination of 1α,25(OH)2D3 and cis-dichlorodiammineplatinum(II) (CDDP) was tested by the colony-forming assay and the measurement of ALP activity and BGP production for differentiating and antitumor effects. The assays revealed that 1α,25(OH)2D3 exerted a dose-related, growth-inhibitory influence. In the colony-forming assay, the 1α,25(OH)2D3-treated colonies were smaller than the untreated colonies. The ALP activity and the BGP production also increased in relation to dose. In the assay in athymic mice, the relative weight of tumors treated with 1α(OH)D3 at 2.5 nmol/kg was significantly smaller than that of the controls, and no side effects were observed in the 1α(OH)D3-treated mice. Marked tumor chondrogenesis was observed in human osteosarcoma treated with 1α(OH)D3 in athymic mice. The combination of 1α,25(OH)2D3 at 10−8 M and CDDP at 2 μg/ml significantly enhanced both the differentiation and the growth inhibition in vitro. Our study apparently is the first demonstration that vitamin D3 metabolites have an antitumor and differentiating effect on human osteosarcoma cells in vitro and in athymic mice. Vitamin D3 should be examined further to discover whether it could be a useful drug in hormonal treatment for human osteosarcomas." @default.
- W2049389638 created "2016-06-24" @default.
- W2049389638 creator A5002896243 @default.
- W2049389638 creator A5022446343 @default.
- W2049389638 creator A5033833528 @default.
- W2049389638 creator A5051109631 @default.
- W2049389638 creator A5056400625 @default.
- W2049389638 date "1993-01-01" @default.
- W2049389638 modified "2023-10-16" @default.
- W2049389638 title "Differentiating and antitumor activities of 1?,25-dihydroxyvitamin D3 in vitro and 1?-hydroxyvitamin D3 in vivo on human osteosarcoma" @default.
- W2049389638 cites W1535885167 @default.
- W2049389638 cites W1540176268 @default.
- W2049389638 cites W1553049056 @default.
- W2049389638 cites W1605030605 @default.
- W2049389638 cites W1748624304 @default.
- W2049389638 cites W1968822620 @default.
- W2049389638 cites W1976202396 @default.
- W2049389638 cites W2004149025 @default.
- W2049389638 cites W2023325346 @default.
- W2049389638 cites W2036088433 @default.
- W2049389638 cites W2041726818 @default.
- W2049389638 cites W2052837225 @default.
- W2049389638 cites W2070704589 @default.
- W2049389638 cites W2076717562 @default.
- W2049389638 cites W2082198146 @default.
- W2049389638 cites W2167132436 @default.
- W2049389638 doi "https://doi.org/10.1002/jor.1100110114" @default.
- W2049389638 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8423514" @default.
- W2049389638 hasPublicationYear "1993" @default.
- W2049389638 type Work @default.
- W2049389638 sameAs 2049389638 @default.
- W2049389638 citedByCount "27" @default.
- W2049389638 countsByYear W20493896382012 @default.
- W2049389638 countsByYear W20493896382013 @default.
- W2049389638 countsByYear W20493896382018 @default.
- W2049389638 crossrefType "journal-article" @default.
- W2049389638 hasAuthorship W2049389638A5002896243 @default.
- W2049389638 hasAuthorship W2049389638A5022446343 @default.
- W2049389638 hasAuthorship W2049389638A5033833528 @default.
- W2049389638 hasAuthorship W2049389638A5051109631 @default.
- W2049389638 hasAuthorship W2049389638A5056400625 @default.
- W2049389638 hasConcept C126322002 @default.
- W2049389638 hasConcept C131075544 @default.
- W2049389638 hasConcept C134018914 @default.
- W2049389638 hasConcept C150903083 @default.
- W2049389638 hasConcept C153911025 @default.
- W2049389638 hasConcept C160160445 @default.
- W2049389638 hasConcept C181199279 @default.
- W2049389638 hasConcept C185592680 @default.
- W2049389638 hasConcept C202751555 @default.
- W2049389638 hasConcept C207001950 @default.
- W2049389638 hasConcept C2777760704 @default.
- W2049389638 hasConcept C2780783641 @default.
- W2049389638 hasConcept C502942594 @default.
- W2049389638 hasConcept C54355233 @default.
- W2049389638 hasConcept C55493867 @default.
- W2049389638 hasConcept C71924100 @default.
- W2049389638 hasConcept C81885089 @default.
- W2049389638 hasConcept C86803240 @default.
- W2049389638 hasConceptScore W2049389638C126322002 @default.
- W2049389638 hasConceptScore W2049389638C131075544 @default.
- W2049389638 hasConceptScore W2049389638C134018914 @default.
- W2049389638 hasConceptScore W2049389638C150903083 @default.
- W2049389638 hasConceptScore W2049389638C153911025 @default.
- W2049389638 hasConceptScore W2049389638C160160445 @default.
- W2049389638 hasConceptScore W2049389638C181199279 @default.
- W2049389638 hasConceptScore W2049389638C185592680 @default.
- W2049389638 hasConceptScore W2049389638C202751555 @default.
- W2049389638 hasConceptScore W2049389638C207001950 @default.
- W2049389638 hasConceptScore W2049389638C2777760704 @default.
- W2049389638 hasConceptScore W2049389638C2780783641 @default.
- W2049389638 hasConceptScore W2049389638C502942594 @default.
- W2049389638 hasConceptScore W2049389638C54355233 @default.
- W2049389638 hasConceptScore W2049389638C55493867 @default.
- W2049389638 hasConceptScore W2049389638C71924100 @default.
- W2049389638 hasConceptScore W2049389638C81885089 @default.
- W2049389638 hasConceptScore W2049389638C86803240 @default.
- W2049389638 hasIssue "1" @default.
- W2049389638 hasLocation W20493896381 @default.
- W2049389638 hasLocation W20493896382 @default.
- W2049389638 hasOpenAccess W2049389638 @default.
- W2049389638 hasPrimaryLocation W20493896381 @default.
- W2049389638 hasRelatedWork W112434655 @default.
- W2049389638 hasRelatedWork W2039448824 @default.
- W2049389638 hasRelatedWork W2135178867 @default.
- W2049389638 hasRelatedWork W2320346276 @default.
- W2049389638 hasRelatedWork W2356699866 @default.
- W2049389638 hasRelatedWork W2363151046 @default.
- W2049389638 hasRelatedWork W2387821449 @default.
- W2049389638 hasRelatedWork W2471807615 @default.
- W2049389638 hasRelatedWork W3178656640 @default.
- W2049389638 hasRelatedWork W4241436983 @default.
- W2049389638 hasVolume "11" @default.
- W2049389638 isParatext "false" @default.
- W2049389638 isRetracted "false" @default.
- W2049389638 magId "2049389638" @default.
- W2049389638 workType "article" @default.