Matches in SemOpenAlex for { <https://semopenalex.org/work/W2049400294> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2049400294 endingPage "80" @default.
- W2049400294 startingPage "74" @default.
- W2049400294 abstract "Cultured inner medullary collecting duct (mIMCD-3) cells express Na+/H+ exchanger isoforms NHE-2 and NHE-1 (Soleimani et al. (1994) J. Biol. Chem. 269, 27973-27978). In the present studies we examined the effect of lethal acid stress on Na+/H+ exchanger activity and isoform expression in mIMCD-3 cells. mIMCD-3 cells were incubated for 10 min with 20 mM ammonium, and exposed to an ammonium-free acidic solution (pH 6.0) for 120 min. Thereafter, cells were recovered and grown in normal culture media. The surviving clones were isolated and subjected to two additional cycles of acid stress. A mutant clone was isolated and characterized for Na+/H+ exchange activity and isoform expression. The mutant mIMCD-3 clone demonstrated significant over-expression of Na+/H+ exchange activity as assessed by acid-stimulated 22Na influx (11.56 nmol/mg protein in mutant vs. 4.06 nmol/mg in parent cells, P < 0.001, n = 4) and sodium-dependent pHi recovery from an acid load (0.55 pH/min in mutant vs. 0.28 pH/min in parent cells, P < 0.01, n = 6). A dose-response inhibition of the exchanger showed that the mutant cells were very sensitive to dimethylamiloride (IC50 158 nM in mutant vs. 889 nM in parent mIMCD-3 cells, P < 0.001). To compare the Na+/H+ exchanger isoforms in mutant and parent mIMCD-3 cells, poly(A)+ RNA was isolated from each group and probed with radiolabeled NHE-1 or NHE-2 cDNA. The expression of NHE-1 mRNA was increased by approximately 100% in mutant cells. The NHE-2 mRNA, on the other hand, was found to be absent in mutant mIMCD-3 cells. Examination of the regulatory mechanisms of the Na+/H+ exchanger isoforms in parent mIMCD-3 cells, which express NHE-2 and NHE-1, and mutant mIMCD-3 cells, which only express NHE-1, would be helpful in elucidating the roles of NHE-2 and NHE-1 in inner medullary collecting duct cells." @default.
- W2049400294 created "2016-06-24" @default.
- W2049400294 creator A5038156580 @default.
- W2049400294 creator A5077938189 @default.
- W2049400294 creator A5077975973 @default.
- W2049400294 date "1995-10-01" @default.
- W2049400294 modified "2023-09-26" @default.
- W2049400294 title "The effect of lethal acid stress on Na+/H+ exchanger isoforms in cultured inner medullary collecting duct cells: deletion of NHE-2 and over expression of NHE-1" @default.
- W2049400294 cites W1482741711 @default.
- W2049400294 cites W1494739644 @default.
- W2049400294 cites W1519805802 @default.
- W2049400294 cites W1554578780 @default.
- W2049400294 cites W1575941030 @default.
- W2049400294 cites W1607594871 @default.
- W2049400294 cites W1976620024 @default.
- W2049400294 cites W1983768617 @default.
- W2049400294 cites W1988081857 @default.
- W2049400294 cites W1992261492 @default.
- W2049400294 cites W2013672165 @default.
- W2049400294 cites W2025100148 @default.
- W2049400294 cites W2031057419 @default.
- W2049400294 cites W2037726983 @default.
- W2049400294 cites W2057595921 @default.
- W2049400294 cites W2092939729 @default.
- W2049400294 cites W2163129250 @default.
- W2049400294 cites W4294216491 @default.
- W2049400294 doi "https://doi.org/10.1016/0005-2736(95)00148-v" @default.
- W2049400294 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7548147" @default.
- W2049400294 hasPublicationYear "1995" @default.
- W2049400294 type Work @default.
- W2049400294 sameAs 2049400294 @default.
- W2049400294 citedByCount "8" @default.
- W2049400294 countsByYear W20494002942022 @default.
- W2049400294 crossrefType "journal-article" @default.
- W2049400294 hasAuthorship W2049400294A5038156580 @default.
- W2049400294 hasAuthorship W2049400294A5077938189 @default.
- W2049400294 hasAuthorship W2049400294A5077975973 @default.
- W2049400294 hasBestOaLocation W20494002941 @default.
- W2049400294 hasConcept C104317684 @default.
- W2049400294 hasConcept C114574056 @default.
- W2049400294 hasConcept C134897140 @default.
- W2049400294 hasConcept C143065580 @default.
- W2049400294 hasConcept C153911025 @default.
- W2049400294 hasConcept C178790620 @default.
- W2049400294 hasConcept C185592680 @default.
- W2049400294 hasConcept C2779870022 @default.
- W2049400294 hasConcept C28406088 @default.
- W2049400294 hasConcept C53345823 @default.
- W2049400294 hasConcept C537181965 @default.
- W2049400294 hasConcept C54355233 @default.
- W2049400294 hasConcept C55493867 @default.
- W2049400294 hasConcept C81089528 @default.
- W2049400294 hasConcept C81885089 @default.
- W2049400294 hasConcept C86803240 @default.
- W2049400294 hasConceptScore W2049400294C104317684 @default.
- W2049400294 hasConceptScore W2049400294C114574056 @default.
- W2049400294 hasConceptScore W2049400294C134897140 @default.
- W2049400294 hasConceptScore W2049400294C143065580 @default.
- W2049400294 hasConceptScore W2049400294C153911025 @default.
- W2049400294 hasConceptScore W2049400294C178790620 @default.
- W2049400294 hasConceptScore W2049400294C185592680 @default.
- W2049400294 hasConceptScore W2049400294C2779870022 @default.
- W2049400294 hasConceptScore W2049400294C28406088 @default.
- W2049400294 hasConceptScore W2049400294C53345823 @default.
- W2049400294 hasConceptScore W2049400294C537181965 @default.
- W2049400294 hasConceptScore W2049400294C54355233 @default.
- W2049400294 hasConceptScore W2049400294C55493867 @default.
- W2049400294 hasConceptScore W2049400294C81089528 @default.
- W2049400294 hasConceptScore W2049400294C81885089 @default.
- W2049400294 hasConceptScore W2049400294C86803240 @default.
- W2049400294 hasIssue "1" @default.
- W2049400294 hasLocation W20494002941 @default.
- W2049400294 hasLocation W20494002942 @default.
- W2049400294 hasOpenAccess W2049400294 @default.
- W2049400294 hasPrimaryLocation W20494002941 @default.
- W2049400294 hasRelatedWork W1840806497 @default.
- W2049400294 hasRelatedWork W1965686949 @default.
- W2049400294 hasRelatedWork W1985861853 @default.
- W2049400294 hasRelatedWork W2004324175 @default.
- W2049400294 hasRelatedWork W2024007126 @default.
- W2049400294 hasRelatedWork W2059843732 @default.
- W2049400294 hasRelatedWork W2062356412 @default.
- W2049400294 hasRelatedWork W2081315501 @default.
- W2049400294 hasRelatedWork W2169334356 @default.
- W2049400294 hasRelatedWork W3151197701 @default.
- W2049400294 hasVolume "1239" @default.
- W2049400294 isParatext "false" @default.
- W2049400294 isRetracted "false" @default.
- W2049400294 magId "2049400294" @default.
- W2049400294 workType "article" @default.