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- W2049536449 abstract "This study attempted to comprehend the interaction mechanisms between DNA and the common minerals in soil such as montmorillonite, kaolinite and goethite at various environmental conditions. The effects of MgCl2 concentrations (0, 1, 10 and 60 mM) and pH (3.0, 5.0, 7.0 and 9.0) on the adsorption of DNA on the examined minerals were investigated by the equilibrium adsorption and direct measurement of adsorption enthalpies. The adsorption isotherms fitted by the Langmuirian model revealed that the maximum capacities and affinities of DNA adsorption on minerals increased with MgCl2 concentrations and decrease of pH. No DNA was adsorbed on montmorillonite at pH 9.0. The values of DNA adsorption enthalpies (ΔHads) ranged from −0.3 to 4.9 kJ g− 1 at different systems. The ΔHads values decreased with increasing MgCl2 concentrations and decreasing pH. The adsorption of DNA on montmorillonite, kaolinite and goethite was an exothermic reaction (−0.3 < ΔHads < − 0.1 kJ g− 1) at 60 mM MgCl2 and pH 3.0, suggesting a more significant electrostatic attraction in the adsorption process. In contrast, DNA adsorption on minerals became endothermic (0.1 < ΔHads < 4.9 kJ g− 1) at 0–10 mM MgCl2 and pH 5.0–9.0 and dehydration effects were considered as the dominant driving forces for DNA adsorption on minerals. The thermodynamic parameters presented in this study have important implications for clarifying the binding mechanisms between DNA and mineral particles in soil and associated environments." @default.
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- W2049536449 date "2006-04-01" @default.
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- W2049536449 title "Microcalorimetric studies of the effects of MgCl2 concentrations and pH on the adsorption of DNA on montmorillonite, kaolinite and goethite" @default.
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- W2049536449 doi "https://doi.org/10.1016/j.clay.2005.11.004" @default.
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