FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2049593316> ?p ?o ?g. }

• W2049593316 endingPage "21593" @default.
• W2049593316 startingPage "21585" @default.
• W2049593316 abstract "Neutrophils express the G protein-coupled N-formyl peptide receptor (FPR) and its homologue FPRL1, whereas monocytes express FPR, FPRL1, and FPRL2, an orphan receptor sharing 83% amino acid identity with FPRL1. FPRL1 is a promiscuous receptor activated by serum amyloid A and by different synthetic peptides, including the hexapeptide Trp-Lys-Tyr-Met-Val-d-Met-NH(2) (WKYMVm). By measuring calcium flux in HL-60 cells transfected with FPR, FPRL1, or FPRL2, we show that WKYMVm activated all three receptors, whereas the l-conformer WKYMVM activated exclusively FPRL1 and FPRL2. The functionality of FPRL2 was further assessed by the ability of HL-60-FPRL2 cells to migrate toward nanomolar concentrations of hexapeptides. The half-maximal effective concentrations of WKYMVM for calcium mobilization in HL-60-FPRL1 and HL-60-FPRL2 cells were 2 and 80 nm, respectively. Those of WKYMVm were 75 pm and 3 nm. The tritiated peptide WK[3,5-(3)H(2)]YMVM bound to FPRL1 (K(D) approximately 160 nm), but not to FPR. The two conformers similarly inhibited binding of (125)I-labeled WKYMVm to FPRL2-expressing cells (IC(50) approximately 2.5-3 micrometer). Metabolic labeling with orthophosphoric acid revealed that FPRL1 was differentially phosphorylated upon addition of the l- or d-conformer, indicating that it induced different conformational changes. In contrast to FPRL1, FPRL2 was already phosphorylated in the absence of agonist and not evenly distributed in the plasma membrane of unstimulated cells. However, both receptors were internalized upon addition of either of the two conformers. Taken together, the results indicate that neutrophils are activated by WKYMVM through FPRL1 and that FPRL2 is a chemotactic receptor transducing signals in myeloid cells." @default.
• W2049593316 created "2016-06-24" @default.
• W2049593316 creator A5004580400 @default.
• W2049593316 creator A5006028451 @default.
• W2049593316 creator A5024403082 @default.
• W2049593316 creator A5038963650 @default.
• W2049593316 creator A5046390385 @default.
• W2049593316 creator A5080658097 @default.
• W2049593316 date "2001-06-01" @default.
• W2049593316 modified "2023-09-27" @default.
• W2049593316 title "The Synthetic Peptide Trp-Lys-Tyr-Met-Val-Met-NH2 Specifically Activates Neutrophils through FPRL1/Lipoxin A4 Receptors and Is an Agonist for the Orphan Monocyte-expressed Chemoattractant Receptor FPRL2" @default.
• W2049593316 cites W1487913306 @default.
• W2049593316 cites W1498425904 @default.
• W2049593316 cites W1568862210 @default.
• W2049593316 cites W1571048371 @default.
• W2049593316 cites W1573414938 @default.
• W2049593316 cites W1586099293 @default.
• W2049593316 cites W1597610606 @default.
• W2049593316 cites W1631597983 @default.
• W2049593316 cites W1846247956 @default.
• W2049593316 cites W1945832201 @default.
• W2049593316 cites W1964603858 @default.
• W2049593316 cites W1968063902 @default.
• W2049593316 cites W1977748538 @default.
• W2049593316 cites W1977986244 @default.
• W2049593316 cites W1980445909 @default.
• W2049593316 cites W1985259310 @default.
• W2049593316 cites W2001647007 @default.
• W2049593316 cites W2004267301 @default.
• W2049593316 cites W2006824774 @default.
• W2049593316 cites W2008243630 @default.
• W2049593316 cites W2013137776 @default.
• W2049593316 cites W2016443795 @default.
• W2049593316 cites W2020174316 @default.
• W2049593316 cites W2031158167 @default.
• W2049593316 cites W2049132647 @default.
• W2049593316 cites W2053202566 @default.
• W2049593316 cites W2054821808 @default.
• W2049593316 cites W2064253903 @default.
• W2049593316 cites W2077883691 @default.
• W2049593316 cites W2081113306 @default.
• W2049593316 cites W2091937188 @default.
• W2049593316 cites W2095441352 @default.
• W2049593316 cites W2098965240 @default.
• W2049593316 cites W2116949924 @default.
• W2049593316 cites W2132342511 @default.
• W2049593316 cites W2139357620 @default.
• W2049593316 cites W2154920085 @default.
• W2049593316 cites W2160013717 @default.
• W2049593316 cites W2169295770 @default.
• W2049593316 cites W2169759699 @default.
• W2049593316 cites W2254553501 @default.
• W2049593316 cites W329126969 @default.
• W2049593316 cites W4231219558 @default.
• W2049593316 cites W4256170791 @default.
• W2049593316 cites W93284168 @default.
• W2049593316 doi "https://doi.org/10.1074/jbc.m007769200" @default.
• W2049593316 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11285256" @default.
• W2049593316 hasPublicationYear "2001" @default.
• W2049593316 type Work @default.
• W2049593316 sameAs 2049593316 @default.
• W2049593316 citedByCount "183" @default.
• W2049593316 countsByYear W20495933162012 @default.
• W2049593316 countsByYear W20495933162013 @default.
• W2049593316 countsByYear W20495933162014 @default.
• W2049593316 countsByYear W20495933162015 @default.
• W2049593316 countsByYear W20495933162016 @default.
• W2049593316 countsByYear W20495933162017 @default.
• W2049593316 countsByYear W20495933162018 @default.
• W2049593316 countsByYear W20495933162019 @default.
• W2049593316 countsByYear W20495933162020 @default.
• W2049593316 countsByYear W20495933162021 @default.
• W2049593316 countsByYear W20495933162022 @default.
• W2049593316 countsByYear W20495933162023 @default.
• W2049593316 crossrefType "journal-article" @default.
• W2049593316 hasAuthorship W2049593316A5004580400 @default.
• W2049593316 hasAuthorship W2049593316A5006028451 @default.
• W2049593316 hasAuthorship W2049593316A5024403082 @default.
• W2049593316 hasAuthorship W2049593316A5038963650 @default.
• W2049593316 hasAuthorship W2049593316A5046390385 @default.
• W2049593316 hasAuthorship W2049593316A5080658097 @default.
• W2049593316 hasBestOaLocation W20495933161 @default.
• W2049593316 hasConcept C104317684 @default.
• W2049593316 hasConcept C153911025 @default.
• W2049593316 hasConcept C170493617 @default.
• W2049593316 hasConcept C185592680 @default.
• W2049593316 hasConcept C203014093 @default.
• W2049593316 hasConcept C2776731609 @default.
• W2049593316 hasConcept C2778938600 @default.
• W2049593316 hasConcept C2779161069 @default.
• W2049593316 hasConcept C2779281246 @default.
• W2049593316 hasConcept C2780415110 @default.
• W2049593316 hasConcept C2781184567 @default.
• W2049593316 hasConcept C54166955 @default.
• W2049593316 hasConcept C55493867 @default.
• W2049593316 hasConcept C58732023 @default.
• W2049593316 hasConcept C86339819 @default.
• W2049593316 hasConcept C86803240 @default.

• next