FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2049649609> ?p ?o ?g. }

• W2049649609 endingPage "155" @default.
• W2049649609 startingPage "141" @default.
• W2049649609 abstract "To detect changes in DNA synthesis during ageing, we compare DNA synthesis in the livers of young and aged rats. As an intermediate between an in vivo system using intact cells and an in vitro system using purified DNA polymerases, isolated nuclei were prepared and used as the machinery for DNA synthesis. The DNA synthesizing capacity of nuclei from regenerating liver was higher than that of nuclei from normal liver and these capacities from liver and regenerating liver were lower in nuclear preparations from aged rats. DNA synthesis using isolated nuclei was stimulated by ATP and the cytoplasmic preparation. The cytoplasmic preparation from regenerating rat liver was found to stimulate DNA synthesis more than the preparation from normal liver. The activity in regenerating liver from young rats was also greater than in that from aged rats. It is well known that DNA replication is inhibited by aphidicolin and DNA repair by ddTTP. We examined the effects of aphidicolin and ddTTP on DNA synthesis using the nuclear system. Surprisingly, the inhibition by aphidicolin was 30% of total DNA synthesis using the nuclear system from young rats. On the other hand, the inhibition by ddTTP was approximately 80%. We measured the sizes of the DNA synthesized in the presence of both inhibitors. DNA synthesis was allowed to proceed for 10 min using isolated nuclei from regenerating liver of young rats and the size of the DNA was determined by sucrose density gradient centrifugation analysis. DNA products appeared in two fractions. Following a chase of 50 min in the presence or absence of aphidicolin, the short DNA product grew larger in both cases, although the amount of DNA in the presence of aphidicolin was approximately 90% that in its absence. In the same experiment using nuclei from aged rats, the amount in the presence of aphidicolin was approximately 60% that in its absence. These results suggest that DNA polymerase beta is closely related to abnormal replication when DNA polymerases alpha and delta are inhibited and that the effect of cytosol on DNA synthesis, as well as the DNA synthetic capacity of isolated nuclei, becomes lower in regenerating rat liver during ageing." @default.
• W2049649609 created "2016-06-24" @default.
• W2049649609 creator A5005607244 @default.
• W2049649609 creator A5011818455 @default.
• W2049649609 creator A5055387930 @default.
• W2049649609 date "2001-02-01" @default.
• W2049649609 modified "2023-09-24" @default.
• W2049649609 title "Differences in DNA synthesis in vitro using isolated nuclei from regenerating livers of young and aged rats" @default.
• W2049649609 cites W1487346902 @default.
• W2049649609 cites W1565624012 @default.
• W2049649609 cites W1592645517 @default.
• W2049649609 cites W1964962486 @default.
• W2049649609 cites W1983236557 @default.
• W2049649609 cites W1983673827 @default.
• W2049649609 cites W1986773920 @default.
• W2049649609 cites W1988989251 @default.
• W2049649609 cites W2004439276 @default.
• W2049649609 cites W2007772649 @default.
• W2049649609 cites W2024143416 @default.
• W2049649609 cites W2024551462 @default.
• W2049649609 cites W2029062218 @default.
• W2049649609 cites W2037177380 @default.
• W2049649609 cites W2042247309 @default.
• W2049649609 cites W2042344764 @default.
• W2049649609 cites W2050359396 @default.
• W2049649609 cites W2052858665 @default.
• W2049649609 cites W2054916685 @default.
• W2049649609 cites W2079111230 @default.
• W2049649609 cites W2079764803 @default.
• W2049649609 cites W2080002581 @default.
• W2049649609 cites W2087249338 @default.
• W2049649609 cites W2090995028 @default.
• W2049649609 cites W2106620902 @default.
• W2049649609 cites W2132826785 @default.
• W2049649609 cites W2135890914 @default.
• W2049649609 cites W2167284883 @default.
• W2049649609 cites W2173897158 @default.
• W2049649609 cites W2177581130 @default.
• W2049649609 cites W2247149472 @default.
• W2049649609 cites W2285466600 @default.
• W2049649609 cites W838635636 @default.
• W2049649609 doi "https://doi.org/10.1016/s0047-6374(00)00226-8" @default.
• W2049649609 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11166354" @default.
• W2049649609 hasPublicationYear "2001" @default.
• W2049649609 type Work @default.
• W2049649609 sameAs 2049649609 @default.
• W2049649609 citedByCount "10" @default.
• W2049649609 countsByYear W20496496092012 @default.
• W2049649609 countsByYear W20496496092014 @default.
• W2049649609 countsByYear W20496496092017 @default.
• W2049649609 countsByYear W20496496092018 @default.
• W2049649609 crossrefType "journal-article" @default.
• W2049649609 hasAuthorship W2049649609A5005607244 @default.
• W2049649609 hasAuthorship W2049649609A5011818455 @default.
• W2049649609 hasAuthorship W2049649609A5055387930 @default.
• W2049649609 hasConcept C104317684 @default.
• W2049649609 hasConcept C115960442 @default.
• W2049649609 hasConcept C153911025 @default.
• W2049649609 hasConcept C202751555 @default.
• W2049649609 hasConcept C207001950 @default.
• W2049649609 hasConcept C24586158 @default.
• W2049649609 hasConcept C2756471 @default.
• W2049649609 hasConcept C2776253266 @default.
• W2049649609 hasConcept C34688535 @default.
• W2049649609 hasConcept C54355233 @default.
• W2049649609 hasConcept C552990157 @default.
• W2049649609 hasConcept C55493867 @default.
• W2049649609 hasConcept C64241487 @default.
• W2049649609 hasConcept C73573662 @default.
• W2049649609 hasConcept C86803240 @default.
• W2049649609 hasConceptScore W2049649609C104317684 @default.
• W2049649609 hasConceptScore W2049649609C115960442 @default.
• W2049649609 hasConceptScore W2049649609C153911025 @default.
• W2049649609 hasConceptScore W2049649609C202751555 @default.
• W2049649609 hasConceptScore W2049649609C207001950 @default.
• W2049649609 hasConceptScore W2049649609C24586158 @default.
• W2049649609 hasConceptScore W2049649609C2756471 @default.
• W2049649609 hasConceptScore W2049649609C2776253266 @default.
• W2049649609 hasConceptScore W2049649609C34688535 @default.
• W2049649609 hasConceptScore W2049649609C54355233 @default.
• W2049649609 hasConceptScore W2049649609C552990157 @default.
• W2049649609 hasConceptScore W2049649609C55493867 @default.
• W2049649609 hasConceptScore W2049649609C64241487 @default.
• W2049649609 hasConceptScore W2049649609C73573662 @default.
• W2049649609 hasConceptScore W2049649609C86803240 @default.
• W2049649609 hasIssue "2" @default.
• W2049649609 hasLocation W20496496091 @default.
• W2049649609 hasLocation W20496496092 @default.
• W2049649609 hasOpenAccess W2049649609 @default.
• W2049649609 hasPrimaryLocation W20496496091 @default.
• W2049649609 hasRelatedWork W1964554956 @default.
• W2049649609 hasRelatedWork W1974828019 @default.
• W2049649609 hasRelatedWork W1985045351 @default.
• W2049649609 hasRelatedWork W2032558243 @default.
• W2049649609 hasRelatedWork W2044974102 @default.
• W2049649609 hasRelatedWork W2049649609 @default.
• W2049649609 hasRelatedWork W2073337599 @default.
• W2049649609 hasRelatedWork W2086786384 @default.

• next