FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2049651868> ?p ?o ?g. }

• W2049651868 endingPage "104" @default.
• W2049651868 startingPage "99" @default.
• W2049651868 abstract "β-Conglycinin is composed of three kinds of subunit: α, α′ and β. A phagocytosis-stimulating peptide sequence (MITLAIPVNKPGR), soymetide, exists in the α′ subunit of β-conglycinin. Met at N terminus of the soymetide is essential for the activity. When Thr at the third residue from N terminus of the soymetide is replaced by Phe or Trp, the phagocytosis-stimulating activity greatly increases (Thr<Phe<Trp). The β subunit does not exhibit the phagocytosis-stimulating activity because the residues corresponding to the first and third residues in the soymetide are Ile and Lys, respectively. In this study, we introduced the phagocytosis-stimulating peptide sequence (Ile→Met, Lys→Thr, Phe, or Trp) into the β subunit after confirmation of the effects of residue replacements by molecular modeling, suggesting that the introduced mutations might not prevent the correct folding. The studies of circular dichroism (CD), gel filtration and differential scanning calorimetry (DSC) of the mutants (I122M/K124T, I122M/K124F, I122M/K124W) expressed in E. coli demonstrated that they folded and self-assembled similarly to the wild type. This was confirmed by X-ray analysis of I122M/K124W crystal where the biggest residue tryptophane was introduced. The three mutants exhibited phagocytosis activities after digestion by trypsin, and the order was the wild type<I122M/K124T<I122M/K124F<I122M/K124W as expected." @default.
• W2049651868 created "2016-06-24" @default.
• W2049651868 creator A5009891435 @default.
• W2049651868 creator A5029241797 @default.
• W2049651868 creator A5043952220 @default.
• W2049651868 creator A5045158548 @default.
• W2049651868 creator A5075464329 @default.
• W2049651868 creator A5084172754 @default.
• W2049651868 date "2003-05-01" @default.
• W2049651868 modified "2023-09-25" @default.
• W2049651868 title "Creation of soybean β-conglycinin β with strong phagocytosis-stimulating activity" @default.
• W2049651868 cites W1539796472 @default.
• W2049651868 cites W1964849842 @default.
• W2049651868 cites W1977899847 @default.
• W2049651868 cites W1983074314 @default.
• W2049651868 cites W1988130533 @default.
• W2049651868 cites W1989190536 @default.
• W2049651868 cites W1994260762 @default.
• W2049651868 cites W1994332698 @default.
• W2049651868 cites W1995017064 @default.
• W2049651868 cites W1997964875 @default.
• W2049651868 cites W1999077012 @default.
• W2049651868 cites W2002133276 @default.
• W2049651868 cites W2003555315 @default.
• W2049651868 cites W2024252792 @default.
• W2049651868 cites W2029359897 @default.
• W2049651868 cites W2052794772 @default.
• W2049651868 cites W2068515260 @default.
• W2049651868 cites W2080476827 @default.
• W2049651868 cites W2089075776 @default.
• W2049651868 cites W2107993128 @default.
• W2049651868 cites W2119728806 @default.
• W2049651868 cites W2147310806 @default.
• W2049651868 doi "https://doi.org/10.1016/s1570-9639(03)00113-4" @default.
• W2049651868 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12758152" @default.
• W2049651868 hasPublicationYear "2003" @default.
• W2049651868 type Work @default.
• W2049651868 sameAs 2049651868 @default.
• W2049651868 citedByCount "32" @default.
• W2049651868 countsByYear W20496518682012 @default.
• W2049651868 countsByYear W20496518682013 @default.
• W2049651868 countsByYear W20496518682014 @default.
• W2049651868 countsByYear W20496518682015 @default.
• W2049651868 countsByYear W20496518682016 @default.
• W2049651868 countsByYear W20496518682017 @default.
• W2049651868 countsByYear W20496518682019 @default.
• W2049651868 countsByYear W20496518682020 @default.
• W2049651868 countsByYear W20496518682022 @default.
• W2049651868 countsByYear W20496518682023 @default.
• W2049651868 crossrefType "journal-article" @default.
• W2049651868 hasAuthorship W2049651868A5009891435 @default.
• W2049651868 hasAuthorship W2049651868A5029241797 @default.
• W2049651868 hasAuthorship W2049651868A5043952220 @default.
• W2049651868 hasAuthorship W2049651868A5045158548 @default.
• W2049651868 hasAuthorship W2049651868A5075464329 @default.
• W2049651868 hasAuthorship W2049651868A5084172754 @default.
• W2049651868 hasConcept C104292427 @default.
• W2049651868 hasConcept C104317684 @default.
• W2049651868 hasConcept C133571119 @default.
• W2049651868 hasConcept C143065580 @default.
• W2049651868 hasConcept C153911025 @default.
• W2049651868 hasConcept C160448771 @default.
• W2049651868 hasConcept C185592680 @default.
• W2049651868 hasConcept C207583985 @default.
• W2049651868 hasConcept C2779281246 @default.
• W2049651868 hasConcept C2781338088 @default.
• W2049651868 hasConcept C55493867 @default.
• W2049651868 hasConcept C86803240 @default.
• W2049651868 hasConcept C95444343 @default.
• W2049651868 hasConceptScore W2049651868C104292427 @default.
• W2049651868 hasConceptScore W2049651868C104317684 @default.
• W2049651868 hasConceptScore W2049651868C133571119 @default.
• W2049651868 hasConceptScore W2049651868C143065580 @default.
• W2049651868 hasConceptScore W2049651868C153911025 @default.
• W2049651868 hasConceptScore W2049651868C160448771 @default.
• W2049651868 hasConceptScore W2049651868C185592680 @default.
• W2049651868 hasConceptScore W2049651868C207583985 @default.
• W2049651868 hasConceptScore W2049651868C2779281246 @default.
• W2049651868 hasConceptScore W2049651868C2781338088 @default.
• W2049651868 hasConceptScore W2049651868C55493867 @default.
• W2049651868 hasConceptScore W2049651868C86803240 @default.
• W2049651868 hasConceptScore W2049651868C95444343 @default.
• W2049651868 hasFunder F4320310760 @default.
• W2049651868 hasFunder F4320311287 @default.
• W2049651868 hasFunder F4320320912 @default.
• W2049651868 hasFunder F4320322266 @default.
• W2049651868 hasIssue "1-2" @default.
• W2049651868 hasLocation W20496518681 @default.
• W2049651868 hasLocation W20496518682 @default.
• W2049651868 hasOpenAccess W2049651868 @default.
• W2049651868 hasPrimaryLocation W20496518681 @default.
• W2049651868 hasRelatedWork W1982348788 @default.
• W2049651868 hasRelatedWork W1996949932 @default.
• W2049651868 hasRelatedWork W2009464217 @default.
• W2049651868 hasRelatedWork W2043130468 @default.
• W2049651868 hasRelatedWork W2085561902 @default.
• W2049651868 hasRelatedWork W2100900129 @default.
• W2049651868 hasRelatedWork W2122465219 @default.

• next