FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2049668890> ?p ?o ?g. }

• W2049668890 endingPage "217" @default.
• W2049668890 startingPage "208" @default.
• W2049668890 abstract "Adenovirus empty capsids are immature intermediates that lack DNA and viral core proteins. Highly purified preparations of empty and full capsids were generated by subjecting purified adenovirus preparations to repeated cesium chloride gradient separations. PAGE results revealed that empty capsids contain at least five bands that correspond to proteins absent from the mature virus proteome. Peptide mapping by matrix-assisted laser desorption/ionization time-of-flight MS revealed that three of these bands correspond to varying forms of L1 52/55 kDa, a protein involved in the encapsidation of the viral DNA. One band at around 31 kDa was found to include precursors to proteins VI and VIII. These precursors correspond to proteins that have not been cleaved by the adenovirus-encoded protease and are not present in the mature full capsids. The precursor to protein VIII (pVIII), a capsid cement protein, is used in this study as a marker in reverse-phased HPLC (RP-HPLC) analyses of adenovirus for the quantitation of empty capsids. A novel calculation method applied to the integration of RP-HPLC chromatograms allowed for the generation of a percentage empty capsid value in a given adenovirus preparation. The percentage empty capsid values generated to date by this method show a high degree of precision and good agreement with a cesium chloride gradient/SDS–PAGE quantitation method of empty capsids. The advantage of this method lies in the accurate, precise, and rapid generation of the percentage of empty capsids in a given purified virus preparation without relying on tedious and time-consuming cesium chloride gradient separations and extractions." @default.
• W2049668890 created "2016-06-24" @default.
• W2049668890 creator A5002646839 @default.
• W2049668890 creator A5013171182 @default.
• W2049668890 creator A5045309577 @default.
• W2049668890 creator A5087767170 @default.
• W2049668890 date "2006-02-01" @default.
• W2049668890 modified "2023-10-17" @default.
• W2049668890 title "Quantitation of adenovirus type 5 empty capsids" @default.
• W2049668890 cites W1574715597 @default.
• W2049668890 cites W1752093409 @default.
• W2049668890 cites W1830251156 @default.
• W2049668890 cites W1911211105 @default.
• W2049668890 cites W1922533605 @default.
• W2049668890 cites W1923099695 @default.
• W2049668890 cites W1966584188 @default.
• W2049668890 cites W1969475775 @default.
• W2049668890 cites W1976594964 @default.
• W2049668890 cites W1983591536 @default.
• W2049668890 cites W1985463101 @default.
• W2049668890 cites W1986957877 @default.
• W2049668890 cites W1992033402 @default.
• W2049668890 cites W1998149067 @default.
• W2049668890 cites W1999193101 @default.
• W2049668890 cites W2015473110 @default.
• W2049668890 cites W2020364299 @default.
• W2049668890 cites W2044338251 @default.
• W2049668890 cites W2045451624 @default.
• W2049668890 cites W2063066512 @default.
• W2049668890 cites W2068733395 @default.
• W2049668890 cites W2069457718 @default.
• W2049668890 cites W2073115309 @default.
• W2049668890 cites W2077591615 @default.
• W2049668890 cites W2093424805 @default.
• W2049668890 cites W2102084617 @default.
• W2049668890 cites W2128108919 @default.
• W2049668890 cites W2129215415 @default.
• W2049668890 cites W2136546144 @default.
• W2049668890 cites W2138505075 @default.
• W2049668890 cites W2138858430 @default.
• W2049668890 cites W2141160974 @default.
• W2049668890 cites W2143969673 @default.
• W2049668890 cites W2154884114 @default.
• W2049668890 cites W2155605080 @default.
• W2049668890 cites W2162897558 @default.
• W2049668890 cites W2170643808 @default.
• W2049668890 cites W2314038177 @default.
• W2049668890 cites W2328476123 @default.
• W2049668890 cites W2334093679 @default.
• W2049668890 cites W2471739061 @default.
• W2049668890 cites W2868963225 @default.
• W2049668890 cites W64861853 @default.
• W2049668890 doi "https://doi.org/10.1016/j.ab.2005.11.014" @default.
• W2049668890 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16360111" @default.
• W2049668890 hasPublicationYear "2006" @default.
• W2049668890 type Work @default.
• W2049668890 sameAs 2049668890 @default.
• W2049668890 citedByCount "31" @default.
• W2049668890 countsByYear W20496688902012 @default.
• W2049668890 countsByYear W20496688902013 @default.
• W2049668890 countsByYear W20496688902014 @default.
• W2049668890 countsByYear W20496688902016 @default.
• W2049668890 countsByYear W20496688902017 @default.
• W2049668890 countsByYear W20496688902018 @default.
• W2049668890 countsByYear W20496688902019 @default.
• W2049668890 countsByYear W20496688902021 @default.
• W2049668890 countsByYear W20496688902022 @default.
• W2049668890 countsByYear W20496688902023 @default.
• W2049668890 crossrefType "journal-article" @default.
• W2049668890 hasAuthorship W2049668890A5002646839 @default.
• W2049668890 hasAuthorship W2049668890A5013171182 @default.
• W2049668890 hasAuthorship W2049668890A5045309577 @default.
• W2049668890 hasAuthorship W2049668890A5087767170 @default.
• W2049668890 hasConcept C104317684 @default.
• W2049668890 hasConcept C104397665 @default.
• W2049668890 hasConcept C153911025 @default.
• W2049668890 hasConcept C159047783 @default.
• W2049668890 hasConcept C185592680 @default.
• W2049668890 hasConcept C202878990 @default.
• W2049668890 hasConcept C2522874641 @default.
• W2049668890 hasConcept C2776954459 @default.
• W2049668890 hasConcept C40767141 @default.
• W2049668890 hasConcept C43617362 @default.
• W2049668890 hasConcept C55493867 @default.
• W2049668890 hasConcept C86803240 @default.
• W2049668890 hasConceptScore W2049668890C104317684 @default.
• W2049668890 hasConceptScore W2049668890C104397665 @default.
• W2049668890 hasConceptScore W2049668890C153911025 @default.
• W2049668890 hasConceptScore W2049668890C159047783 @default.
• W2049668890 hasConceptScore W2049668890C185592680 @default.
• W2049668890 hasConceptScore W2049668890C202878990 @default.
• W2049668890 hasConceptScore W2049668890C2522874641 @default.
• W2049668890 hasConceptScore W2049668890C2776954459 @default.
• W2049668890 hasConceptScore W2049668890C40767141 @default.
• W2049668890 hasConceptScore W2049668890C43617362 @default.
• W2049668890 hasConceptScore W2049668890C55493867 @default.
• W2049668890 hasConceptScore W2049668890C86803240 @default.
• W2049668890 hasIssue "2" @default.

• next