FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2049706996> ?p ?o ?g. }

• W2049706996 endingPage "172" @default.
• W2049706996 startingPage "167" @default.
• W2049706996 abstract "Tumour necrosis factor (TNF) is an important cytokine that induces an inflammatory response predominantly through the TNF receptor-1 (TNFR1). A crucial strategy for the treatment of many autoimmune diseases, therefore, is to block the binding of TNF to TNFR1. We previously identified a TNFR1-selective antagonistic mutant TNF (R1antTNF) from a phage library containing six randomized amino acid residues at the receptor-binding site (amino acids 84-89). Two R1antTNFs, R1antTNF-T2 (A84S, V85T, S86T, Y87H, Q88N and T89Q) and R1antTNF-T8 (A84T, V85P, S86A, Y87I, Q88N and T89R), were successfully isolated from this library. Here, we analysed R1antTNF-T8 using surface plasmon resonance spectroscopy and X-ray crystallography to determine the mechanism underlying the antagonistic activity of R1antTNF. The kinetic association/dissociation parameters of R1antTNF-T8 were higher than those of wild-type TNF, indicating more rapid bond dissociation. X-ray crystallographic analysis suggested that the binding mode of the T89R mutation changed from a hydrophobic to an electrostatic interaction, which may be responsible for the antagonistic behaviour of R1antTNF. Knowledge of these structure-function relationships will facilitate the design of novel TNF inhibitors based on the cytokine structure." @default.
• W2049706996 created "2016-06-24" @default.
• W2049706996 creator A5008699926 @default.
• W2049706996 creator A5016781421 @default.
• W2049706996 creator A5019680889 @default.
• W2049706996 creator A5028835943 @default.
• W2049706996 creator A5030423707 @default.
• W2049706996 creator A5032281643 @default.
• W2049706996 creator A5032740050 @default.
• W2049706996 creator A5036754333 @default.
• W2049706996 creator A5044202216 @default.
• W2049706996 creator A5072978499 @default.
• W2049706996 creator A5086059561 @default.
• W2049706996 creator A5086856467 @default.
• W2049706996 creator A5090975978 @default.
• W2049706996 date "2009-04-22" @default.
• W2049706996 modified "2023-09-26" @default.
• W2049706996 title "Fast Binding Kinetics and Conserved 3D Structure Underlie the Antagonistic Activity of Mutant TNF: Useful Information for Designing Artificial Proteo-Antagonists" @default.
• W2049706996 cites W1499288756 @default.
• W2049706996 cites W1517287118 @default.
• W2049706996 cites W1539796472 @default.
• W2049706996 cites W1977233832 @default.
• W2049706996 cites W1981306008 @default.
• W2049706996 cites W1988949024 @default.
• W2049706996 cites W1994342014 @default.
• W2049706996 cites W1995017064 @default.
• W2049706996 cites W2000640473 @default.
• W2049706996 cites W2013083986 @default.
• W2049706996 cites W2016300251 @default.
• W2049706996 cites W2031144635 @default.
• W2049706996 cites W2038535814 @default.
• W2049706996 cites W2067387741 @default.
• W2049706996 cites W2068343030 @default.
• W2049706996 cites W2091627659 @default.
• W2049706996 cites W2095013915 @default.
• W2049706996 cites W2108743612 @default.
• W2049706996 cites W2112807945 @default.
• W2049706996 cites W2158329969 @default.
• W2049706996 cites W3120153401 @default.
• W2049706996 doi "https://doi.org/10.1093/jb/mvp065" @default.
• W2049706996 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19386778" @default.
• W2049706996 hasPublicationYear "2009" @default.
• W2049706996 type Work @default.
• W2049706996 sameAs 2049706996 @default.
• W2049706996 citedByCount "13" @default.
• W2049706996 countsByYear W20497069962012 @default.
• W2049706996 countsByYear W20497069962013 @default.
• W2049706996 countsByYear W20497069962014 @default.
• W2049706996 countsByYear W20497069962015 @default.
• W2049706996 countsByYear W20497069962017 @default.
• W2049706996 countsByYear W20497069962020 @default.
• W2049706996 countsByYear W20497069962022 @default.
• W2049706996 crossrefType "journal-article" @default.
• W2049706996 hasAuthorship W2049706996A5008699926 @default.
• W2049706996 hasAuthorship W2049706996A5016781421 @default.
• W2049706996 hasAuthorship W2049706996A5019680889 @default.
• W2049706996 hasAuthorship W2049706996A5028835943 @default.
• W2049706996 hasAuthorship W2049706996A5030423707 @default.
• W2049706996 hasAuthorship W2049706996A5032281643 @default.
• W2049706996 hasAuthorship W2049706996A5032740050 @default.
• W2049706996 hasAuthorship W2049706996A5036754333 @default.
• W2049706996 hasAuthorship W2049706996A5044202216 @default.
• W2049706996 hasAuthorship W2049706996A5072978499 @default.
• W2049706996 hasAuthorship W2049706996A5086059561 @default.
• W2049706996 hasAuthorship W2049706996A5086856467 @default.
• W2049706996 hasAuthorship W2049706996A5090975978 @default.
• W2049706996 hasConcept C102931765 @default.
• W2049706996 hasConcept C104317684 @default.
• W2049706996 hasConcept C106847996 @default.
• W2049706996 hasConcept C107824862 @default.
• W2049706996 hasConcept C12554922 @default.
• W2049706996 hasConcept C143065580 @default.
• W2049706996 hasConcept C147789679 @default.
• W2049706996 hasConcept C152900378 @default.
• W2049706996 hasConcept C155672457 @default.
• W2049706996 hasConcept C170493617 @default.
• W2049706996 hasConcept C171250308 @default.
• W2049706996 hasConcept C17991360 @default.
• W2049706996 hasConcept C185592680 @default.
• W2049706996 hasConcept C192562407 @default.
• W2049706996 hasConcept C203014093 @default.
• W2049706996 hasConcept C2778690821 @default.
• W2049706996 hasConcept C515207424 @default.
• W2049706996 hasConcept C54355233 @default.
• W2049706996 hasConcept C55493867 @default.
• W2049706996 hasConcept C71240020 @default.
• W2049706996 hasConcept C86803240 @default.
• W2049706996 hasConcept C90260826 @default.
• W2049706996 hasConceptScore W2049706996C102931765 @default.
• W2049706996 hasConceptScore W2049706996C104317684 @default.
• W2049706996 hasConceptScore W2049706996C106847996 @default.
• W2049706996 hasConceptScore W2049706996C107824862 @default.
• W2049706996 hasConceptScore W2049706996C12554922 @default.
• W2049706996 hasConceptScore W2049706996C143065580 @default.
• W2049706996 hasConceptScore W2049706996C147789679 @default.
• W2049706996 hasConceptScore W2049706996C152900378 @default.
• W2049706996 hasConceptScore W2049706996C155672457 @default.
• W2049706996 hasConceptScore W2049706996C170493617 @default.

• next