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- W2049812933 abstract "The molecular karyotypes of 36 clones derived from 8 strains of Leishmania infantum were examined by pulsed-field gel electrophoresis. Although there appeared to be a high degree of genetic relatedness between the clones and the parent strain, a limited degree of polymorphism was noted in 50% of the clones, expressed mainly as the presence of an additional chromosome or as a chromosome size modification. Repeated subcloning in one strain showed that chromosomal rearrangements could occur during the cloning process. Chromosome homologies were examined by Southern analysis with chromosome-specific DNA probes. The results suggest a disomy for some chromosomes, but cannot exclude aneuploidy. The mechanisms possibly leading to such heterogeneity are discussed: they could involve frequent DNA amplification/deletion, and imply a ‘mosaic’ structure of the cultured strains or clones, with different individuals possessing differently sized versions of the same chromosomes." @default.
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- W2049812933 date "1990-04-01" @default.
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- W2049812933 title "Interclonal variations in molecular karyotype in Leishmania infantum imply a ‘mosaic’ strain structure" @default.
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- W2049812933 doi "https://doi.org/10.1016/0166-6851(90)90079-2" @default.
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