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• W2049822801 abstract "Pan1 is an actin patch‐associated protein involved in endocytosis. Our studies revealed that in oleate‐grown cells Pan1 is located in the nucleus as well as in patches. One of three putative nuclear localization signals (NLS) of Pan1, NLS2, directed β‐galactosidase (β‐gal) to the nucleus. However, GFP‐Pan1 886−1219 , containing NLS2, was found in the cytoplasm indicating that it may contain a nuclear export signal (NES). A putative Pan1 NES, overlapping with NLS3, re‐addressed NLS H2B ‐NES/NLS3‐β‐gal from the nucleus to the cytoplasm. Inactivation of the NES allowed NLS3 to be effective. Thus, Pan1 contains functional NLSs and a NES and appears to shuttle in certain circumstances." @default.
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• W2049822801 date "2007-10-29" @default.
• W2049822801 modified "2023-10-18" @default.
• W2049822801 title "Characterization of nuclear localization and nuclear export signals of yeast actin-binding protein Pan1" @default.
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• W2049822801 doi "https://doi.org/10.1016/j.febslet.2007.10.036" @default.
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