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- W2049841857 abstract "A noncompetitive enzyme-linked immunosorbent assay (ELISA) for apolipoprotein A-II (ApoA-II) was developed. Microtiter plates were coated with affinity purified antibodies to ApoA-II. After incubation with human plasma, the amount of ApoA-II bound to the coated plate was determined with peroxidase-labeled antibodies to ApoA-II. When pure ApoA-II or delipidated reference plasma was used as standard, a single step delipidation was required in order to unmask some antigenic sites of ApoA-II. However, the underestimated ApoA-II values in untreated samples were shown to be corrected by using intact reference plasma as secondary standard. The average concentration of ApoA-II in normolipidemic plasma was 0.376 g/l." @default.
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- W2049841857 date "1986-12-01" @default.
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- W2049841857 title "Quantitative Determination of Human Plasma Apolipoprotein A-II by a Non Competitive Enzyme-Linked Immunosorbent Assay" @default.
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- W2049841857 doi "https://doi.org/10.1080/01971528608060473" @default.
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