Matches in SemOpenAlex for { <https://semopenalex.org/work/W2049842852> ?p ?o ?g. }
- W2049842852 endingPage "457" @default.
- W2049842852 startingPage "443" @default.
- W2049842852 abstract "Single cell suspensions of human keratinocytes when seeded onto floating three-dimensional gels constructed with type I collagen form a tissue resembling epidermis. These morphogenetic events occur in a serum-free environment in the absence of fibroblasts. Light and transmission electron microscopy show that cells form a basal layer plus suprabasilar cell layers corresponding to the stratum spinosum, stratum granulosum, and stratum corneum. The suprabasilar keratinocyte layers show morphologies which resemble intact skin in which cells are connected by desmosomes and contain intermediate filaments and keratohyalin-fillagrin granules. The basal cell layer differs from skin in vivo in that there is no connection to a basement membrane via hemidesmosomes. Cells in the basal layers are polarized as evidenced by the secretion of type IV collagen, heparan sulfate proteoglycans, and laminin at the cell membrane interface with the collagen gel. These proteins are not organized into a cytological basement membrane. Bullous pemphigoid antigen, a protein component of hemidesmosomes, is synthesized by basal keratinocytes, but like the basement membrane proteins it is not incorporated into a definable cytological structure. Keratinocytes in the basal and suprabasilar layers also synthesize α2β1 integrins. The mechanisms of keratinocyte adhesion to the gel may be through the interactions of this cell surface receptor with laminin and type IV collagen synthesized by the cell and/or direct interactions between the receptor and type I collagen within the gel. This in vitro experimental system is a useful model for defining the molecular events which control the formation and turnover of basement membranes and the mechanisms by which keratinocytes adhere to type I collagen when sheets of keratinocytes are used clinically for wound coverage." @default.
- W2049842852 created "2016-06-24" @default.
- W2049842852 creator A5019046192 @default.
- W2049842852 creator A5022950318 @default.
- W2049842852 creator A5028705322 @default.
- W2049842852 creator A5038009943 @default.
- W2049842852 date "1991-08-01" @default.
- W2049842852 modified "2023-10-18" @default.
- W2049842852 title "Human keratinocytes cultured on collagen gels form an epidermis which synthesizes bullous pemphigoid antigens and ?2?1 integrins and secretes laminin, type IV collagen, and heparan sulfate proteoglycan at the basal cell surface" @default.
- W2049842852 cites W1498888463 @default.
- W2049842852 cites W157732207 @default.
- W2049842852 cites W1628719417 @default.
- W2049842852 cites W175266247 @default.
- W2049842852 cites W1967888499 @default.
- W2049842852 cites W1970533599 @default.
- W2049842852 cites W1972034563 @default.
- W2049842852 cites W1976702022 @default.
- W2049842852 cites W1977064819 @default.
- W2049842852 cites W1978873659 @default.
- W2049842852 cites W1985091308 @default.
- W2049842852 cites W1985938777 @default.
- W2049842852 cites W1992104579 @default.
- W2049842852 cites W1992338530 @default.
- W2049842852 cites W2004633058 @default.
- W2049842852 cites W2024700555 @default.
- W2049842852 cites W2034128763 @default.
- W2049842852 cites W2034282182 @default.
- W2049842852 cites W2034363769 @default.
- W2049842852 cites W2040541853 @default.
- W2049842852 cites W2042665858 @default.
- W2049842852 cites W2046112878 @default.
- W2049842852 cites W2047423042 @default.
- W2049842852 cites W2054468033 @default.
- W2049842852 cites W2055135391 @default.
- W2049842852 cites W2056914328 @default.
- W2049842852 cites W2059332937 @default.
- W2049842852 cites W2062926268 @default.
- W2049842852 cites W2063227608 @default.
- W2049842852 cites W2073172113 @default.
- W2049842852 cites W2084258562 @default.
- W2049842852 cites W2090905928 @default.
- W2049842852 cites W2095489990 @default.
- W2049842852 cites W2102193002 @default.
- W2049842852 cites W2102271673 @default.
- W2049842852 cites W2110586785 @default.
- W2049842852 cites W2135057126 @default.
- W2049842852 cites W2137949057 @default.
- W2049842852 cites W2138539863 @default.
- W2049842852 cites W2152921630 @default.
- W2049842852 cites W2153768968 @default.
- W2049842852 cites W2161140556 @default.
- W2049842852 cites W2169406360 @default.
- W2049842852 cites W2171740885 @default.
- W2049842852 cites W613699236 @default.
- W2049842852 cites W2036189250 @default.
- W2049842852 doi "https://doi.org/10.1016/0014-4827(91)90395-b" @default.
- W2049842852 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2070826" @default.
- W2049842852 hasPublicationYear "1991" @default.
- W2049842852 type Work @default.
- W2049842852 sameAs 2049842852 @default.
- W2049842852 citedByCount "31" @default.
- W2049842852 countsByYear W20498428522014 @default.
- W2049842852 countsByYear W20498428522015 @default.
- W2049842852 countsByYear W20498428522021 @default.
- W2049842852 crossrefType "journal-article" @default.
- W2049842852 hasAuthorship W2049842852A5019046192 @default.
- W2049842852 hasAuthorship W2049842852A5022950318 @default.
- W2049842852 hasAuthorship W2049842852A5028705322 @default.
- W2049842852 hasAuthorship W2049842852A5038009943 @default.
- W2049842852 hasConcept C119524353 @default.
- W2049842852 hasConcept C147075424 @default.
- W2049842852 hasConcept C1491633281 @default.
- W2049842852 hasConcept C189165786 @default.
- W2049842852 hasConcept C195687474 @default.
- W2049842852 hasConcept C202751555 @default.
- W2049842852 hasConcept C2776193487 @default.
- W2049842852 hasConcept C2777074287 @default.
- W2049842852 hasConcept C2777107211 @default.
- W2049842852 hasConcept C2778370115 @default.
- W2049842852 hasConcept C2779814568 @default.
- W2049842852 hasConcept C3060791 @default.
- W2049842852 hasConcept C54355233 @default.
- W2049842852 hasConcept C55493867 @default.
- W2049842852 hasConcept C86803240 @default.
- W2049842852 hasConcept C95444343 @default.
- W2049842852 hasConceptScore W2049842852C119524353 @default.
- W2049842852 hasConceptScore W2049842852C147075424 @default.
- W2049842852 hasConceptScore W2049842852C1491633281 @default.
- W2049842852 hasConceptScore W2049842852C189165786 @default.
- W2049842852 hasConceptScore W2049842852C195687474 @default.
- W2049842852 hasConceptScore W2049842852C202751555 @default.
- W2049842852 hasConceptScore W2049842852C2776193487 @default.
- W2049842852 hasConceptScore W2049842852C2777074287 @default.
- W2049842852 hasConceptScore W2049842852C2777107211 @default.
- W2049842852 hasConceptScore W2049842852C2778370115 @default.
- W2049842852 hasConceptScore W2049842852C2779814568 @default.
- W2049842852 hasConceptScore W2049842852C3060791 @default.
- W2049842852 hasConceptScore W2049842852C54355233 @default.