FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2049843437> ?p ?o ?g. }

• W2049843437 endingPage "1210" @default.
• W2049843437 startingPage "1201" @default.
• W2049843437 abstract "We previously discovered a cellular isoform of titin (originally named T-protein) colocalized with myosin II in the terminal web domain of the chicken intestinal epithelial cell brush border cytoskeleton (Eilertsen, K.J., and T.C.S. Keller. 1992. J. Cell Biol. 119:549-557). Here, we demonstrate that cellular titin also colocalizes with myosin II filaments in stress fibers and organizes a similar array of myosin II filaments in vitro. To investigate interactions between cellular titin and myosin in vitro, we purified both proteins from isolated intestinal epithelial cell brush borders by a combination of gel filtration and hydroxyapatite column chromatography. Electron microscopy of brush border myosin bipolar filaments assembled in the presence and absence of cellular titin revealed a cellular titin-dependent side-by-side and end-to-end alignment of the filaments into highly ordered arrays. Immunogold labeling confirmed cellular titin association with the filament arrays. Under similar assembly conditions, purified chicken pectoralis muscle titin formed much less regular aggregates of muscle myosin bipolar filaments. Sucrose density gradient analyses of both cellular and muscle titin-myosin supramolecular arrays demonstrated that the cellular titin and myosin isoforms coassembled with a myosin/titin ratio of approximately 25:1, whereas the muscle isoforms coassembled with a myosin:titin ratio of approximately 38:1. No coassembly aggregates were found when cellular myosin was assembled in the presence of muscle titin or when muscle myosin was assembled in the presence of cellular titin. Our results demonstrate that cellular titin can organize an isoform-specific association of myosin II bipolar filaments and support the possibility that cellular titin is a key organizing component of the brush border and other myosin II-containing cytoskeletal structures including stress fibers." @default.
• W2049843437 created "2016-06-24" @default.
• W2049843437 creator A5020651924 @default.
• W2049843437 creator A5056796827 @default.
• W2049843437 creator A5065094180 @default.
• W2049843437 date "1994-09-01" @default.
• W2049843437 modified "2023-10-13" @default.
• W2049843437 title "Cellular titin localization in stress fibers and interaction with myosin II filaments in vitro." @default.
• W2049843437 cites W102016573 @default.
• W2049843437 cites W1266384774 @default.
• W2049843437 cites W1504890289 @default.
• W2049843437 cites W1925983352 @default.
• W2049843437 cites W1953411904 @default.
• W2049843437 cites W1958794775 @default.
• W2049843437 cites W1970507036 @default.
• W2049843437 cites W1979965298 @default.
• W2049843437 cites W1988331525 @default.
• W2049843437 cites W1990902768 @default.
• W2049843437 cites W1991884643 @default.
• W2049843437 cites W1994138436 @default.
• W2049843437 cites W1995670995 @default.
• W2049843437 cites W2009867098 @default.
• W2049843437 cites W2016628557 @default.
• W2049843437 cites W2018289835 @default.
• W2049843437 cites W2023375965 @default.
• W2049843437 cites W2038783550 @default.
• W2049843437 cites W2044532304 @default.
• W2049843437 cites W2047208889 @default.
• W2049843437 cites W2051760300 @default.
• W2049843437 cites W2057516353 @default.
• W2049843437 cites W2059908035 @default.
• W2049843437 cites W2071114580 @default.
• W2049843437 cites W2073879684 @default.
• W2049843437 cites W2075738691 @default.
• W2049843437 cites W2081740469 @default.
• W2049843437 cites W2087573052 @default.
• W2049843437 cites W2088051308 @default.
• W2049843437 cites W2092145907 @default.
• W2049843437 cites W2100837269 @default.
• W2049843437 cites W2103345293 @default.
• W2049843437 cites W2108386137 @default.
• W2049843437 cites W2117832434 @default.
• W2049843437 cites W2118202513 @default.
• W2049843437 cites W2119460480 @default.
• W2049843437 cites W2139292873 @default.
• W2049843437 cites W2148583233 @default.
• W2049843437 cites W2155480562 @default.
• W2049843437 cites W2161912679 @default.
• W2049843437 cites W2172852338 @default.
• W2049843437 cites W2403005311 @default.
• W2049843437 doi "https://doi.org/10.1083/jcb.126.5.1201" @default.
• W2049843437 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2120159" @default.
• W2049843437 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8063857" @default.
• W2049843437 hasPublicationYear "1994" @default.
• W2049843437 type Work @default.
• W2049843437 sameAs 2049843437 @default.
• W2049843437 citedByCount "50" @default.
• W2049843437 countsByYear W20498434372013 @default.
• W2049843437 countsByYear W20498434372015 @default.
• W2049843437 countsByYear W20498434372020 @default.
• W2049843437 countsByYear W20498434372021 @default.
• W2049843437 crossrefType "journal-article" @default.
• W2049843437 hasAuthorship W2049843437A5020651924 @default.
• W2049843437 hasAuthorship W2049843437A5056796827 @default.
• W2049843437 hasAuthorship W2049843437A5065094180 @default.
• W2049843437 hasBestOaLocation W20498434371 @default.
• W2049843437 hasConcept C12554922 @default.
• W2049843437 hasConcept C142669718 @default.
• W2049843437 hasConcept C1491633281 @default.
• W2049843437 hasConcept C207200792 @default.
• W2049843437 hasConcept C37140074 @default.
• W2049843437 hasConcept C55493867 @default.
• W2049843437 hasConcept C68731436 @default.
• W2049843437 hasConcept C6997183 @default.
• W2049843437 hasConcept C8035138 @default.
• W2049843437 hasConcept C86803240 @default.
• W2049843437 hasConcept C90671521 @default.
• W2049843437 hasConcept C95444343 @default.
• W2049843437 hasConceptScore W2049843437C12554922 @default.
• W2049843437 hasConceptScore W2049843437C142669718 @default.
• W2049843437 hasConceptScore W2049843437C1491633281 @default.
• W2049843437 hasConceptScore W2049843437C207200792 @default.
• W2049843437 hasConceptScore W2049843437C37140074 @default.
• W2049843437 hasConceptScore W2049843437C55493867 @default.
• W2049843437 hasConceptScore W2049843437C68731436 @default.
• W2049843437 hasConceptScore W2049843437C6997183 @default.
• W2049843437 hasConceptScore W2049843437C8035138 @default.
• W2049843437 hasConceptScore W2049843437C86803240 @default.
• W2049843437 hasConceptScore W2049843437C90671521 @default.
• W2049843437 hasConceptScore W2049843437C95444343 @default.
• W2049843437 hasIssue "5" @default.
• W2049843437 hasLocation W20498434371 @default.
• W2049843437 hasLocation W20498434372 @default.
• W2049843437 hasLocation W20498434373 @default.
• W2049843437 hasLocation W20498434374 @default.
• W2049843437 hasOpenAccess W2049843437 @default.
• W2049843437 hasPrimaryLocation W20498434371 @default.
• W2049843437 hasRelatedWork W1971161141 @default.

• next