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• W2049882365 abstract "Cultivation-based microbiological methods are a gold standard for monitoring of airborne micro-organisms to determine the occupational exposure levels or transmission paths of a particular infectious agent. Some highly contagious microorganisms are not easily culturable but it is becoming evident that cultivation and molecular methods are complementary and in these cases highly relevant. We report a simple and efficient method for sampling and analyzing airborne bacteria with an impactor-type high-flow-rate portable air sampler, currently used for monitoring culturable bacteria or fungi. A method is reported for extraction of nucleic acids from impacted cells without prior cultivation and using agarose as a sampling matrix. The DNA extraction efficiency was determined in spiked samples and, samples taken from a wastewater treatment plant and an alpine area. The abundance, diversity and quantity of total bacteria were analysed by a quantitative polymerase chain reaction, and by construction and analysis of clone libraries. The method does not interfere with downstream PCR analysis and can cover the gap between traditional culture and molecular techniques of bioaerosol monitoring." @default.
• W2049882365 created "2016-06-24" @default.
• W2049882365 creator A5011434869 @default.
• W2049882365 creator A5014044868 @default.
• W2049882365 date "2013-12-20" @default.
• W2049882365 modified "2023-09-27" @default.
• W2049882365 title "The Efficient Method for Simultaneous Monitoring of the Culturable as Well as Nonculturable Airborne Microorganisms" @default.
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• W2049882365 doi "https://doi.org/10.1371/journal.pone.0082186" @default.
• W2049882365 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3869663" @default.
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