FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2049940642> ?p ?o ?g. }

• W2049940642 abstract "Cellular movement in response to external stimuli is fundamental to many cellular processes including wound healing, inflammation and the response to infection. A common method to measure chemotaxis is the Boyden chamber assay, in which cells and chemoattractant are separated by a porous membrane. As cells migrate through the membrane toward the chemoattractant, they adhere to the underside of the membrane, or fall into the underlying media, and are subsequently stained and visually counted (1). In this method, cells are exposed to a steep and transient chemoattractant gradient, which is thought to be a poor representation of gradients found in tissues (2). Another assay system, the under-agarose chemotaxis assay, (3, 4) measures cell movement across a solid substrate in a thin aqueous film that forms under the agarose layer. The gradient that develops in the agarose is shallow and is thought to be an appropriate representation of naturally occurring gradients. Chemotaxis can be evaluated by microscopic imaging of the distance traveled. Both the Boyden chamber assay and the under-agarose assay are usually configured as endpoint assays. The automated ECIS/Taxis system combines the under-agarose approach with Electric Cell-substrate Impedance Sensing (ECIS) (5, 6). In this assay, target electrodes are located in each of 8 chambers. A large counter-electrode runs through each of the 8 chambers (Figure 2). Each chamber is filled with agarose and two small wells are the cut in the agarose on either side of the target electrode. One well is filled with the test cell population, while the other holds the sources of diffusing chemoattractant (Figure 3). Current passed through the system can be used to determine the change in resistance that occurs as cells pass over the target electrode. Cells on the target electrode increase the resistance of the system (6). In addition, rapid fluctuations in the resistance represent changes in the interactions of cells with the electrode surface and are indicative of ongoing cellular shape changes. The ECIS/Taxis system can measure movement of the cell population in real-time over extended periods of time, but is also sensitive enough to detect the arrival of a single cell at the target electrode. Dictyostelium discoidium is known to migrate in the presence of a folate gradient (7, 8) and its chemotactic response can be accurately measured by ECIS/Taxis (9). Leukocyte chemotaxis, in response to SDF1α and to chemotaxis antagonists has also been measured with ECIS/Taxis (10, 11). An example of the leukocyte response to SDF1α is shown in Figure 1." @default.
• W2049940642 created "2016-06-24" @default.
• W2049940642 creator A5035365624 @default.
• W2049940642 creator A5053963155 @default.
• W2049940642 creator A5054601712 @default.
• W2049940642 creator A5090554845 @default.
• W2049940642 date "2012-04-01" @default.
• W2049940642 modified "2023-10-17" @default.
• W2049940642 title "Measurement of Cellular Chemotaxis with ECIS/Taxis" @default.
• W2049940642 cites W107153115 @default.
• W2049940642 cites W1502626182 @default.
• W2049940642 cites W1758183764 @default.
• W2049940642 cites W1789275826 @default.
• W2049940642 cites W1819732475 @default.
• W2049940642 cites W1980462928 @default.
• W2049940642 cites W2024319008 @default.
• W2049940642 cites W2033949877 @default.
• W2049940642 cites W2036464893 @default.
• W2049940642 cites W2043090087 @default.
• W2049940642 cites W2050662100 @default.
• W2049940642 cites W2105610793 @default.
• W2049940642 cites W2117228071 @default.
• W2049940642 cites W2125509960 @default.
• W2049940642 cites W2162885959 @default.
• W2049940642 doi "https://doi.org/10.3791/3840" @default.
• W2049940642 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3460541" @default.
• W2049940642 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22491349" @default.
• W2049940642 hasPublicationYear "2012" @default.
• W2049940642 type Work @default.
• W2049940642 sameAs 2049940642 @default.
• W2049940642 citedByCount "9" @default.
• W2049940642 countsByYear W20499406422014 @default.
• W2049940642 countsByYear W20499406422015 @default.
• W2049940642 countsByYear W20499406422016 @default.
• W2049940642 countsByYear W20499406422017 @default.
• W2049940642 countsByYear W20499406422021 @default.
• W2049940642 countsByYear W20499406422022 @default.
• W2049940642 crossrefType "journal-article" @default.
• W2049940642 hasAuthorship W2049940642A5035365624 @default.
• W2049940642 hasAuthorship W2049940642A5053963155 @default.
• W2049940642 hasAuthorship W2049940642A5054601712 @default.
• W2049940642 hasAuthorship W2049940642A5090554845 @default.
• W2049940642 hasBestOaLocation W20499406422 @default.
• W2049940642 hasConcept C12554922 @default.
• W2049940642 hasConcept C170493617 @default.
• W2049940642 hasConcept C185592680 @default.
• W2049940642 hasConcept C2778476535 @default.
• W2049940642 hasConcept C2779866169 @default.
• W2049940642 hasConcept C2908647359 @default.
• W2049940642 hasConcept C54166955 @default.
• W2049940642 hasConcept C55493867 @default.
• W2049940642 hasConcept C71924100 @default.
• W2049940642 hasConcept C86803240 @default.
• W2049940642 hasConcept C95444343 @default.
• W2049940642 hasConcept C99454951 @default.
• W2049940642 hasConceptScore W2049940642C12554922 @default.
• W2049940642 hasConceptScore W2049940642C170493617 @default.
• W2049940642 hasConceptScore W2049940642C185592680 @default.
• W2049940642 hasConceptScore W2049940642C2778476535 @default.
• W2049940642 hasConceptScore W2049940642C2779866169 @default.
• W2049940642 hasConceptScore W2049940642C2908647359 @default.
• W2049940642 hasConceptScore W2049940642C54166955 @default.
• W2049940642 hasConceptScore W2049940642C55493867 @default.
• W2049940642 hasConceptScore W2049940642C71924100 @default.
• W2049940642 hasConceptScore W2049940642C86803240 @default.
• W2049940642 hasConceptScore W2049940642C95444343 @default.
• W2049940642 hasConceptScore W2049940642C99454951 @default.
• W2049940642 hasIssue "62" @default.
• W2049940642 hasLocation W20499406421 @default.
• W2049940642 hasLocation W20499406422 @default.
• W2049940642 hasLocation W20499406423 @default.
• W2049940642 hasOpenAccess W2049940642 @default.
• W2049940642 hasPrimaryLocation W20499406421 @default.
• W2049940642 hasRelatedWork W1805484436 @default.
• W2049940642 hasRelatedWork W2016285437 @default.
• W2049940642 hasRelatedWork W2024823771 @default.
• W2049940642 hasRelatedWork W2048537083 @default.
• W2049940642 hasRelatedWork W2064823163 @default.
• W2049940642 hasRelatedWork W2121397463 @default.
• W2049940642 hasRelatedWork W2321137488 @default.
• W2049940642 hasRelatedWork W2335496580 @default.
• W2049940642 hasRelatedWork W2398903576 @default.
• W2049940642 hasRelatedWork W2789308123 @default.
• W2049940642 isParatext "false" @default.
• W2049940642 isRetracted "false" @default.
• W2049940642 magId "2049940642" @default.
• W2049940642 workType "article" @default.