FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2050004685> ?p ?o ?g. }

• W2050004685 endingPage "10960" @default.
• W2050004685 startingPage "10950" @default.
• W2050004685 abstract "Functional consequences of constraining β-Gal in bidimensional space were studied at defined molecular packing densities and constant topology. Langmuir−Blodgett films, LB15 and LB35 composed of dipalmitoyl phosphatidylcholine and K. lactis β-Gal, were obtained by transferring Langmuir films (L) initially packed at 15 and 35 mN/m, respectively, to alkylated glasses. The β-Gal-monolayer binding equilibrium, mainly the adsorption rate and affinity, depended on the initial monolayer’s surface pressure (lower for higher πi). At πi = 15 and 35 mN/m, the surface excess (Γ) followed downward parabolic and power-law tendencies, respectively, as a function of subphase protein concentration. Γ values in L roughly reflected the protein surface density chemically determined in LBs (0−7.5 ng/mm2 at πi = 0−35 mN/m and [β-Gal]subphase = 0−100 μg/mL). The β-Gal-catalyzed hydrolysis of o-nitrophenyl-galactopyranoside showed a Michaelian kinetics in solution as well as in LB15. KM, KM,LB15, Vmax, and Vmax,LB15 were 5.15 ± 2.2 and 9.25 ± 6 mM and 39.63 and 0.0096 ± 0.0027 μmol/min/mg protein, respectively. The sigmoidal kinetics observed with LB35 was evaluated by Hill’s model (K0.5 = 9.55 ± 0.4 mM, Vmax,35 = 0.0021 μmol/min/mg protein, Hill coefficient n = 9) and Savageau’s fractal model (fractal constant Kf = 9.84 mM; reaction order for the substrate gs = 9.06 and for the enzyme ge = 0.62). Fractal reaction orders would reflect the fractal organization of the environment, demonstrated by AFM images, more than the molecularity of the reaction. Particular dynamics of the protein−lipid structural coupling in each molecular packing condition would have led to the different kinetic responses." @default.
• W2050004685 created "2016-06-24" @default.
• W2050004685 creator A5058886673 @default.
• W2050004685 creator A5071774347 @default.
• W2050004685 creator A5085893405 @default.
• W2050004685 creator A5091188566 @default.
• W2050004685 date "2008-09-04" @default.
• W2050004685 modified "2023-10-01" @default.
• W2050004685 title "Molecular Packing Tunes the Activity of <i>Kluyveromyces lactis</i> β-Galactosidase Incorporated in Langmuir−Blodgett Films" @default.
• W2050004685 cites W1775749144 @default.
• W2050004685 cites W1972546400 @default.
• W2050004685 cites W1981260337 @default.
• W2050004685 cites W1981984118 @default.
• W2050004685 cites W1985400133 @default.
• W2050004685 cites W1987541472 @default.
• W2050004685 cites W1988124106 @default.
• W2050004685 cites W1995715964 @default.
• W2050004685 cites W1998526986 @default.
• W2050004685 cites W2006188688 @default.
• W2050004685 cites W2008722942 @default.
• W2050004685 cites W2011650323 @default.
• W2050004685 cites W2014430242 @default.
• W2050004685 cites W2017849876 @default.
• W2050004685 cites W2020975071 @default.
• W2050004685 cites W2022987595 @default.
• W2050004685 cites W2023748701 @default.
• W2050004685 cites W2033108852 @default.
• W2050004685 cites W2033525556 @default.
• W2050004685 cites W2039696862 @default.
• W2050004685 cites W2044034494 @default.
• W2050004685 cites W2057876985 @default.
• W2050004685 cites W2063443549 @default.
• W2050004685 cites W2067132840 @default.
• W2050004685 cites W2072881082 @default.
• W2050004685 cites W2076841135 @default.
• W2050004685 cites W2078206416 @default.
• W2050004685 cites W2081096260 @default.
• W2050004685 cites W2081871457 @default.
• W2050004685 cites W2083597481 @default.
• W2050004685 cites W2090983022 @default.
• W2050004685 cites W2110961644 @default.
• W2050004685 cites W2115355246 @default.
• W2050004685 cites W2118422627 @default.
• W2050004685 cites W2126469537 @default.
• W2050004685 cites W2131187223 @default.
• W2050004685 cites W2142124608 @default.
• W2050004685 cites W2147796031 @default.
• W2050004685 cites W2161174290 @default.
• W2050004685 cites W2407452144 @default.
• W2050004685 doi "https://doi.org/10.1021/la801679m" @default.
• W2050004685 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18767827" @default.
• W2050004685 hasPublicationYear "2008" @default.
• W2050004685 type Work @default.
• W2050004685 sameAs 2050004685 @default.
• W2050004685 citedByCount "18" @default.
• W2050004685 countsByYear W20500046852012 @default.
• W2050004685 countsByYear W20500046852013 @default.
• W2050004685 countsByYear W20500046852014 @default.
• W2050004685 countsByYear W20500046852015 @default.
• W2050004685 countsByYear W20500046852018 @default.
• W2050004685 countsByYear W20500046852019 @default.
• W2050004685 countsByYear W20500046852020 @default.
• W2050004685 countsByYear W20500046852023 @default.
• W2050004685 crossrefType "journal-article" @default.
• W2050004685 hasAuthorship W2050004685A5058886673 @default.
• W2050004685 hasAuthorship W2050004685A5071774347 @default.
• W2050004685 hasAuthorship W2050004685A5085893405 @default.
• W2050004685 hasAuthorship W2050004685A5091188566 @default.
• W2050004685 hasConcept C111368507 @default.
• W2050004685 hasConcept C113196181 @default.
• W2050004685 hasConcept C115958267 @default.
• W2050004685 hasConcept C121332964 @default.
• W2050004685 hasConcept C12143843 @default.
• W2050004685 hasConcept C127313418 @default.
• W2050004685 hasConcept C147789679 @default.
• W2050004685 hasConcept C148898269 @default.
• W2050004685 hasConcept C150394285 @default.
• W2050004685 hasConcept C162711632 @default.
• W2050004685 hasConcept C178790620 @default.
• W2050004685 hasConcept C180235531 @default.
• W2050004685 hasConcept C185592680 @default.
• W2050004685 hasConcept C2777289219 @default.
• W2050004685 hasConcept C43617362 @default.
• W2050004685 hasConcept C55493867 @default.
• W2050004685 hasConcept C62520636 @default.
• W2050004685 hasConcept C7070889 @default.
• W2050004685 hasConcept C71240020 @default.
• W2050004685 hasConcept C8010536 @default.
• W2050004685 hasConcept C93391505 @default.
• W2050004685 hasConcept C94412978 @default.
• W2050004685 hasConceptScore W2050004685C111368507 @default.
• W2050004685 hasConceptScore W2050004685C113196181 @default.
• W2050004685 hasConceptScore W2050004685C115958267 @default.
• W2050004685 hasConceptScore W2050004685C121332964 @default.
• W2050004685 hasConceptScore W2050004685C12143843 @default.
• W2050004685 hasConceptScore W2050004685C127313418 @default.
• W2050004685 hasConceptScore W2050004685C147789679 @default.
• W2050004685 hasConceptScore W2050004685C148898269 @default.

• next