FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2050055227> ?p ?o ?g. }

• W2050055227 abstract "Surface membrane proteins of virulent RH strain and tissue cyst-forming Fukaya strain of Toxoplasma gondii were analyzed by SDS-polyacrylamide gel electrophoresis after LPO-catalyzed surface iodination and lectin blotting, then identified the zoite-specific antigens. Prior to the analyses, purification of RH tachyzoites from mouse peritoneal exudate and of Fukaya bradyzoites from mouse brain tissues were performed by centrifugation on the discontinuous Percoll density-gradient. Tachyzoites were obtained at the interface of 50 per cent and 60 per cent Percoll solution and brain cysts were harvested at the interfaces of 40-50 per cent and 50-60 per cent, then bradyzoites were obtained by treating the cysts with hypertonic solution. The LPO-catalyzed iodination detected 15 KDa and 14 KDa proteins of bradyzoites and 30 KDa protein of tachyzoites as major bands with several other minor bands. But Con A blotting revealed some bands of 200 K-50 KDa glycoproteins of bradyzoites and 52 KDa band as major and minor bands of 33 K-20 KDa of tachyzoites. Phytohemagglutinin did not detect any band in the two forms. EITB with anti-Fukaya antibody and anti-RH antibody revealed cross-reactivities between the two forms. Despite the cross-reactivity, anti-Fukaya antibody reacted with 15 KDa band of bradyzoites specifically and, anti-RH antibody with 52 KDa, 30 KDa, and 25 KDa bands of tachyzoites, respectively. It was identified that 15 KDa protein in bradyzoite, which was not a glycoprotein, was a major membrane protein with sufficient antigenicity, and in the case of tachyzoite, 52 KDa surface glycoprotein (gp52) with specific antigenicity might be added to the major surface protein, p30." @default.
• W2050055227 created "2016-06-24" @default.
• W2050055227 creator A5028495189 @default.
• W2050055227 creator A5044270992 @default.
• W2050055227 creator A5051695205 @default.
• W2050055227 date "1988-01-01" @default.
• W2050055227 modified "2023-09-25" @default.
• W2050055227 title "Membrane proteins and their antigenicity of Toxoplasma gondii" @default.
• W2050055227 cites W1878568934 @default.
• W2050055227 cites W1964599133 @default.
• W2050055227 cites W1990975460 @default.
• W2050055227 cites W1991442980 @default.
• W2050055227 cites W1995855588 @default.
• W2050055227 cites W2020291403 @default.
• W2050055227 cites W2031708336 @default.
• W2050055227 cites W2035885771 @default.
• W2050055227 cites W2050951459 @default.
• W2050055227 cites W2057253149 @default.
• W2050055227 cites W2066485735 @default.
• W2050055227 cites W2069487431 @default.
• W2050055227 cites W2083634695 @default.
• W2050055227 cites W2100837269 @default.
• W2050055227 cites W2101108802 @default.
• W2050055227 cites W2103647613 @default.
• W2050055227 cites W2104173407 @default.
• W2050055227 cites W2105489590 @default.
• W2050055227 cites W2127212175 @default.
• W2050055227 cites W2165393039 @default.
• W2050055227 cites W2170078117 @default.
• W2050055227 cites W2320926837 @default.
• W2050055227 cites W2321090111 @default.
• W2050055227 doi "https://doi.org/10.3347/kjp.1988.26.3.155" @default.
• W2050055227 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12811042" @default.
• W2050055227 hasPublicationYear "1988" @default.
• W2050055227 type Work @default.
• W2050055227 sameAs 2050055227 @default.
• W2050055227 citedByCount "3" @default.
• W2050055227 crossrefType "journal-article" @default.
• W2050055227 hasAuthorship W2050055227A5028495189 @default.
• W2050055227 hasAuthorship W2050055227A5044270992 @default.
• W2050055227 hasAuthorship W2050055227A5051695205 @default.
• W2050055227 hasBestOaLocation W20500552271 @default.
• W2050055227 hasConcept C101660505 @default.
• W2050055227 hasConcept C104317684 @default.
• W2050055227 hasConcept C108625454 @default.
• W2050055227 hasConcept C116084860 @default.
• W2050055227 hasConcept C144647389 @default.
• W2050055227 hasConcept C147483822 @default.
• W2050055227 hasConcept C153911025 @default.
• W2050055227 hasConcept C159654299 @default.
• W2050055227 hasConcept C181199279 @default.
• W2050055227 hasConcept C185592680 @default.
• W2050055227 hasConcept C203014093 @default.
• W2050055227 hasConcept C2776415932 @default.
• W2050055227 hasConcept C2778128430 @default.
• W2050055227 hasConcept C2779026604 @default.
• W2050055227 hasConcept C41625074 @default.
• W2050055227 hasConcept C48679268 @default.
• W2050055227 hasConcept C55493867 @default.
• W2050055227 hasConcept C7623868 @default.
• W2050055227 hasConcept C85265743 @default.
• W2050055227 hasConcept C86803240 @default.
• W2050055227 hasConceptScore W2050055227C101660505 @default.
• W2050055227 hasConceptScore W2050055227C104317684 @default.
• W2050055227 hasConceptScore W2050055227C108625454 @default.
• W2050055227 hasConceptScore W2050055227C116084860 @default.
• W2050055227 hasConceptScore W2050055227C144647389 @default.
• W2050055227 hasConceptScore W2050055227C147483822 @default.
• W2050055227 hasConceptScore W2050055227C153911025 @default.
• W2050055227 hasConceptScore W2050055227C159654299 @default.
• W2050055227 hasConceptScore W2050055227C181199279 @default.
• W2050055227 hasConceptScore W2050055227C185592680 @default.
• W2050055227 hasConceptScore W2050055227C203014093 @default.
• W2050055227 hasConceptScore W2050055227C2776415932 @default.
• W2050055227 hasConceptScore W2050055227C2778128430 @default.
• W2050055227 hasConceptScore W2050055227C2779026604 @default.
• W2050055227 hasConceptScore W2050055227C41625074 @default.
• W2050055227 hasConceptScore W2050055227C48679268 @default.
• W2050055227 hasConceptScore W2050055227C55493867 @default.
• W2050055227 hasConceptScore W2050055227C7623868 @default.
• W2050055227 hasConceptScore W2050055227C85265743 @default.
• W2050055227 hasConceptScore W2050055227C86803240 @default.
• W2050055227 hasLocation W20500552271 @default.
• W2050055227 hasLocation W20500552272 @default.
• W2050055227 hasOpenAccess W2050055227 @default.
• W2050055227 hasPrimaryLocation W20500552271 @default.
• W2050055227 hasRelatedWork W101372637 @default.
• W2050055227 hasRelatedWork W1568218075 @default.
• W2050055227 hasRelatedWork W1874309304 @default.
• W2050055227 hasRelatedWork W1974483800 @default.
• W2050055227 hasRelatedWork W1981024153 @default.
• W2050055227 hasRelatedWork W1986517634 @default.
• W2050055227 hasRelatedWork W1986909105 @default.
• W2050055227 hasRelatedWork W1992464030 @default.
• W2050055227 hasRelatedWork W1998615549 @default.
• W2050055227 hasRelatedWork W1999275828 @default.
• W2050055227 hasRelatedWork W2006363933 @default.
• W2050055227 hasRelatedWork W2013222317 @default.
• W2050055227 hasRelatedWork W2039391755 @default.
• W2050055227 hasRelatedWork W2056951034 @default.

• next