FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2050067585> ?p ?o ?g. }

• W2050067585 abstract "Abstract Background Potassium channels are essential for cell survival and participate in the regulation of cell membrane potential and electrochemical gradients. During its lifecycle, Plasmodium falciparum parasites must successfully traverse widely diverse environmental milieus, in which K + channel function is likely to be critical. Dramatically differing conditions will be presented to the parasite in the mosquito mid-gut, red blood cell (RBC) cytosol and the human circulatory system. Methods In silico sequence analyses identified two open-reading frames in the P. falciparum genome that are predicted to encode for proteins with high homology to K + channels. To further analyse these putative channels, specific antisera were generated and used in immunoblot and immunofluorescence analyses of P. falciparum -infected RBCs. Recombinant genome methods in cultured P. falciparum were used to create genetic knock outs of each K + channel gene to asses the importance of their expression. Results Immunoblot and IFA analyses confirmed the expression of the two putative P. falciparum K + channels (PfK1 and PfK2). PfK1 is expressed in all asexual stage parasites, predominantly in late stages and localizes to the RBC membrane. Conversely, PfK2 is predominantly expressed in late schizont and merozoite stage parasites and remains primarily localized to the parasite. Repeated attempts to knockout PfK1 and PfK2 expression by targeted gene disruption proved unsuccessful despite evidence of recombinant gene integration, indicating that pfk1 and pfk2 are apparently refractory to genetic disruption. Conclusion Putative K + channel proteins PfK1 and PfK2 are expressed in cultured P. falciparum parasites with differing spatial and temporal patterns. Eventual functional characterization of these channels may reveal future pharmacological targets." @default.
• W2050067585 created "2016-06-24" @default.
• W2050067585 creator A5042341113 @default.
• W2050067585 creator A5059027982 @default.
• W2050067585 creator A5072276610 @default.
• W2050067585 creator A5091421770 @default.
• W2050067585 date "2008-01-24" @default.
• W2050067585 modified "2023-10-18" @default.
• W2050067585 title "Characterization of two putative potassium channels in Plasmodium falciparum" @default.
• W2050067585 cites W1550911363 @default.
• W2050067585 cites W1551825440 @default.
• W2050067585 cites W1969503178 @default.
• W2050067585 cites W1973541716 @default.
• W2050067585 cites W1981723999 @default.
• W2050067585 cites W1983602710 @default.
• W2050067585 cites W1987902824 @default.
• W2050067585 cites W1987927734 @default.
• W2050067585 cites W2018581247 @default.
• W2050067585 cites W2020251171 @default.
• W2050067585 cites W2026483075 @default.
• W2050067585 cites W2037350416 @default.
• W2050067585 cites W2048536141 @default.
• W2050067585 cites W2051343250 @default.
• W2050067585 cites W2055527040 @default.
• W2050067585 cites W2061032623 @default.
• W2050067585 cites W2084321932 @default.
• W2050067585 cites W2086437923 @default.
• W2050067585 cites W2097432311 @default.
• W2050067585 cites W2099705774 @default.
• W2050067585 cites W2112047656 @default.
• W2050067585 cites W2127020680 @default.
• W2050067585 cites W2128252987 @default.
• W2050067585 cites W2130096579 @default.
• W2050067585 cites W2136690044 @default.
• W2050067585 cites W2161366128 @default.
• W2050067585 cites W2162195177 @default.
• W2050067585 cites W2164312318 @default.
• W2050067585 cites W2164713083 @default.
• W2050067585 cites W2344075384 @default.
• W2050067585 cites W2415590762 @default.
• W2050067585 doi "https://doi.org/10.1186/1475-2875-7-19" @default.
• W2050067585 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2263067" @default.
• W2050067585 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18218136" @default.
• W2050067585 hasPublicationYear "2008" @default.
• W2050067585 type Work @default.
• W2050067585 sameAs 2050067585 @default.
• W2050067585 citedByCount "30" @default.
• W2050067585 countsByYear W20500675852012 @default.
• W2050067585 countsByYear W20500675852013 @default.
• W2050067585 countsByYear W20500675852014 @default.
• W2050067585 countsByYear W20500675852015 @default.
• W2050067585 countsByYear W20500675852016 @default.
• W2050067585 countsByYear W20500675852017 @default.
• W2050067585 countsByYear W20500675852018 @default.
• W2050067585 countsByYear W20500675852019 @default.
• W2050067585 countsByYear W20500675852020 @default.
• W2050067585 countsByYear W20500675852021 @default.
• W2050067585 countsByYear W20500675852022 @default.
• W2050067585 crossrefType "journal-article" @default.
• W2050067585 hasAuthorship W2050067585A5042341113 @default.
• W2050067585 hasAuthorship W2050067585A5059027982 @default.
• W2050067585 hasAuthorship W2050067585A5072276610 @default.
• W2050067585 hasAuthorship W2050067585A5091421770 @default.
• W2050067585 hasBestOaLocation W20500675851 @default.
• W2050067585 hasConcept C104317684 @default.
• W2050067585 hasConcept C108305142 @default.
• W2050067585 hasConcept C12554922 @default.
• W2050067585 hasConcept C141231307 @default.
• W2050067585 hasConcept C153911025 @default.
• W2050067585 hasConcept C159047783 @default.
• W2050067585 hasConcept C203014093 @default.
• W2050067585 hasConcept C2775905019 @default.
• W2050067585 hasConcept C2778048844 @default.
• W2050067585 hasConcept C2778371730 @default.
• W2050067585 hasConcept C40767141 @default.
• W2050067585 hasConcept C54355233 @default.
• W2050067585 hasConcept C83743174 @default.
• W2050067585 hasConcept C86803240 @default.
• W2050067585 hasConcept C95444343 @default.
• W2050067585 hasConceptScore W2050067585C104317684 @default.
• W2050067585 hasConceptScore W2050067585C108305142 @default.
• W2050067585 hasConceptScore W2050067585C12554922 @default.
• W2050067585 hasConceptScore W2050067585C141231307 @default.
• W2050067585 hasConceptScore W2050067585C153911025 @default.
• W2050067585 hasConceptScore W2050067585C159047783 @default.
• W2050067585 hasConceptScore W2050067585C203014093 @default.
• W2050067585 hasConceptScore W2050067585C2775905019 @default.
• W2050067585 hasConceptScore W2050067585C2778048844 @default.
• W2050067585 hasConceptScore W2050067585C2778371730 @default.
• W2050067585 hasConceptScore W2050067585C40767141 @default.
• W2050067585 hasConceptScore W2050067585C54355233 @default.
• W2050067585 hasConceptScore W2050067585C83743174 @default.
• W2050067585 hasConceptScore W2050067585C86803240 @default.
• W2050067585 hasConceptScore W2050067585C95444343 @default.
• W2050067585 hasIssue "1" @default.
• W2050067585 hasLocation W20500675851 @default.
• W2050067585 hasLocation W20500675852 @default.
• W2050067585 hasLocation W20500675853 @default.
• W2050067585 hasLocation W20500675854 @default.
• W2050067585 hasLocation W20500675855 @default.

• next