Matches in SemOpenAlex for { <https://semopenalex.org/work/W2050080739> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2050080739 endingPage "517" @default.
- W2050080739 startingPage "510" @default.
- W2050080739 abstract "During the course of isolating, characterizing, and cloning estrogen and 3-hydroxysteroid sulfotransferases from the guinea pig adrenal gland, it was noted that cytosolic preparations from this tissue would also sulfonate testosterone. Therefore, we set out to isolate and clone the enzyme that performs this reaction. Testosterone sulfotransferase (TST) was isolated from the guinea pig adrenal by using the standard procedures of ion exchange, affinity, and high-performance liquid chromatography. When purified, TST was examined by liquid-phase nondenaturing isoelectric focusing, it was found that the TST activity profile completely overlapped with the activity profile of the 3alpha-hydroxysteroid sulfotransferase (3alphaHST) isoform, but not the 3beta-hydroxysteroid sulfotransferase (3betaHST) isoform. This finding was further investigated by overexpressing the cDNAs for 3alphaHST and 3betaHST in Escherichia coli and examining the expressed proteins for TST activity. This experiment confirmed that 3alphaHST does indeed function as a TST. In addition, 3alphaHST was also found to sulfonate estradiol but not estrone, a finding that further suggested that 3alphaHST may function as a general 17beta-hydroxysteroid sulfotransferase." @default.
- W2050080739 created "2016-06-24" @default.
- W2050080739 creator A5004531345 @default.
- W2050080739 creator A5018697776 @default.
- W2050080739 creator A5067482851 @default.
- W2050080739 date "1999-08-01" @default.
- W2050080739 modified "2023-10-14" @default.
- W2050080739 title "Testosterone sulfotransferase: Evidence in the guinea pig that this reaction is carried out by 3α-hydroxysteroid sulfotransferase" @default.
- W2050080739 cites W1492298514 @default.
- W2050080739 cites W1565684791 @default.
- W2050080739 cites W1970140089 @default.
- W2050080739 cites W1972684203 @default.
- W2050080739 cites W1980295415 @default.
- W2050080739 cites W1997266569 @default.
- W2050080739 cites W2002615049 @default.
- W2050080739 cites W2003965848 @default.
- W2050080739 cites W2004129996 @default.
- W2050080739 cites W2027558790 @default.
- W2050080739 cites W2035838507 @default.
- W2050080739 cites W2053534100 @default.
- W2050080739 cites W2063042295 @default.
- W2050080739 cites W2092107040 @default.
- W2050080739 cites W2100837269 @default.
- W2050080739 cites W2101108802 @default.
- W2050080739 cites W2102357667 @default.
- W2050080739 cites W2110542699 @default.
- W2050080739 cites W2121983824 @default.
- W2050080739 cites W2127314264 @default.
- W2050080739 cites W2163670995 @default.
- W2050080739 cites W2171142088 @default.
- W2050080739 cites W2465729181 @default.
- W2050080739 cites W4233825604 @default.
- W2050080739 cites W4235559323 @default.
- W2050080739 cites W4239142147 @default.
- W2050080739 cites W4240048848 @default.
- W2050080739 cites W4243378940 @default.
- W2050080739 cites W4245936763 @default.
- W2050080739 cites W4250779219 @default.
- W2050080739 cites W4293247451 @default.
- W2050080739 cites W2064957240 @default.
- W2050080739 doi "https://doi.org/10.1016/s0039-128x(99)00027-6" @default.
- W2050080739 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10493595" @default.
- W2050080739 hasPublicationYear "1999" @default.
- W2050080739 type Work @default.
- W2050080739 sameAs 2050080739 @default.
- W2050080739 citedByCount "2" @default.
- W2050080739 countsByYear W20500807392019 @default.
- W2050080739 crossrefType "journal-article" @default.
- W2050080739 hasAuthorship W2050080739A5004531345 @default.
- W2050080739 hasAuthorship W2050080739A5018697776 @default.
- W2050080739 hasAuthorship W2050080739A5067482851 @default.
- W2050080739 hasConcept C134018914 @default.
- W2050080739 hasConcept C181199279 @default.
- W2050080739 hasConcept C185592680 @default.
- W2050080739 hasConcept C2775904581 @default.
- W2050080739 hasConcept C2776317432 @default.
- W2050080739 hasConcept C2777164284 @default.
- W2050080739 hasConcept C2778656385 @default.
- W2050080739 hasConcept C2778683662 @default.
- W2050080739 hasConcept C2778815084 @default.
- W2050080739 hasConcept C2779279991 @default.
- W2050080739 hasConcept C55493867 @default.
- W2050080739 hasConcept C71315377 @default.
- W2050080739 hasConcept C86803240 @default.
- W2050080739 hasConceptScore W2050080739C134018914 @default.
- W2050080739 hasConceptScore W2050080739C181199279 @default.
- W2050080739 hasConceptScore W2050080739C185592680 @default.
- W2050080739 hasConceptScore W2050080739C2775904581 @default.
- W2050080739 hasConceptScore W2050080739C2776317432 @default.
- W2050080739 hasConceptScore W2050080739C2777164284 @default.
- W2050080739 hasConceptScore W2050080739C2778656385 @default.
- W2050080739 hasConceptScore W2050080739C2778683662 @default.
- W2050080739 hasConceptScore W2050080739C2778815084 @default.
- W2050080739 hasConceptScore W2050080739C2779279991 @default.
- W2050080739 hasConceptScore W2050080739C55493867 @default.
- W2050080739 hasConceptScore W2050080739C71315377 @default.
- W2050080739 hasConceptScore W2050080739C86803240 @default.
- W2050080739 hasIssue "8" @default.
- W2050080739 hasLocation W20500807391 @default.
- W2050080739 hasLocation W20500807392 @default.
- W2050080739 hasOpenAccess W2050080739 @default.
- W2050080739 hasPrimaryLocation W20500807391 @default.
- W2050080739 hasRelatedWork W12313613 @default.
- W2050080739 hasRelatedWork W1980292711 @default.
- W2050080739 hasRelatedWork W2000716065 @default.
- W2050080739 hasRelatedWork W2019374191 @default.
- W2050080739 hasRelatedWork W2027038085 @default.
- W2050080739 hasRelatedWork W2028617796 @default.
- W2050080739 hasRelatedWork W2050080739 @default.
- W2050080739 hasRelatedWork W2122706524 @default.
- W2050080739 hasRelatedWork W2148958861 @default.
- W2050080739 hasRelatedWork W2739806842 @default.
- W2050080739 hasVolume "64" @default.
- W2050080739 isParatext "false" @default.
- W2050080739 isRetracted "false" @default.
- W2050080739 magId "2050080739" @default.
- W2050080739 workType "article" @default.