Matches in SemOpenAlex for { <https://semopenalex.org/work/W2050081230> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2050081230 endingPage "7493" @default.
- W2050081230 startingPage "7484" @default.
- W2050081230 abstract "The Chinese hamster ovary cell (CHO) glycosylation mutant, LEC14, was previously selected for resistance to pea lectin (Pisum sativum agglutinin) and shown to behave dominantly. The lectin resistance properties of LEC14 cells are related to, but distinct from, those of LEC18, a dominant Chinese hamster ovary mutant that synthesizes complex N-glycans with a novel O-6-linked GlcNAc residue in the core region (Raju, T. S., Ray. M., and Stanley, P.(1995) J. Biol. Chem. 270, 30294-30302). Detailed structural studies of a complex N-glycan fraction from LEC14 cells have revealed yet another novel modification of the core region. [3H]Glc-labeled LEC14 cellular glycopeptides were desialylated, and the fraction that did not bind to concanavalin A-Sepharose was found to have an increased proportion of species that bound to tomato-agarose, and to ricin-agarose. 1H NMR spectroscopy and methylation linkage analysis of the tomato and ricin-bound fractions purified from ~1010 LEC14 cells showed they were complex N-glycans containing a 2,3,6-trisubstituted core Man residue. To examine the core region more closely, these N-glycans were digested with mixtures of β-D-galactosidases and N-acetyl-β-D-glucosaminidases to obtain core glycopeptides. The latter were largely unbound by concanavalin A-Sepharose or pea lectin-agarose. 1H NMR spectroscopy and electrospray ionization-mass spectrometry showed that the LEC14 core glycopeptides contain a new GlcNAc residue that substitutes the core β(1,4)-Man residue at O-2 to give the following novel, N-linked core structure." @default.
- W2050081230 created "2016-06-24" @default.
- W2050081230 creator A5069308432 @default.
- W2050081230 creator A5085697781 @default.
- W2050081230 date "1996-03-01" @default.
- W2050081230 modified "2023-10-16" @default.
- W2050081230 title "LEC14, a Dominant Chinese Hamster Ovary Glycosylation Mutant Expresses Complex N-Glycans with a New N-Acetylglucosamine Residue in the Core Region" @default.
- W2050081230 doi "https://doi.org/10.1074/jbc.271.13.7484" @default.
- W2050081230 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8631778" @default.
- W2050081230 hasPublicationYear "1996" @default.
- W2050081230 type Work @default.
- W2050081230 sameAs 2050081230 @default.
- W2050081230 citedByCount "15" @default.
- W2050081230 countsByYear W20500812302012 @default.
- W2050081230 crossrefType "journal-article" @default.
- W2050081230 hasAuthorship W2050081230A5069308432 @default.
- W2050081230 hasAuthorship W2050081230A5085697781 @default.
- W2050081230 hasBestOaLocation W20500812301 @default.
- W2050081230 hasConcept C104317684 @default.
- W2050081230 hasConcept C108625454 @default.
- W2050081230 hasConcept C143065580 @default.
- W2050081230 hasConcept C170493617 @default.
- W2050081230 hasConcept C175656101 @default.
- W2050081230 hasConcept C181199279 @default.
- W2050081230 hasConcept C185592680 @default.
- W2050081230 hasConcept C206212055 @default.
- W2050081230 hasConcept C2777313579 @default.
- W2050081230 hasConcept C2778815778 @default.
- W2050081230 hasConcept C2781338088 @default.
- W2050081230 hasConcept C55493867 @default.
- W2050081230 hasConcept C71240020 @default.
- W2050081230 hasConcept C86803240 @default.
- W2050081230 hasConcept C95444343 @default.
- W2050081230 hasConceptScore W2050081230C104317684 @default.
- W2050081230 hasConceptScore W2050081230C108625454 @default.
- W2050081230 hasConceptScore W2050081230C143065580 @default.
- W2050081230 hasConceptScore W2050081230C170493617 @default.
- W2050081230 hasConceptScore W2050081230C175656101 @default.
- W2050081230 hasConceptScore W2050081230C181199279 @default.
- W2050081230 hasConceptScore W2050081230C185592680 @default.
- W2050081230 hasConceptScore W2050081230C206212055 @default.
- W2050081230 hasConceptScore W2050081230C2777313579 @default.
- W2050081230 hasConceptScore W2050081230C2778815778 @default.
- W2050081230 hasConceptScore W2050081230C2781338088 @default.
- W2050081230 hasConceptScore W2050081230C55493867 @default.
- W2050081230 hasConceptScore W2050081230C71240020 @default.
- W2050081230 hasConceptScore W2050081230C86803240 @default.
- W2050081230 hasConceptScore W2050081230C95444343 @default.
- W2050081230 hasIssue "13" @default.
- W2050081230 hasLocation W20500812301 @default.
- W2050081230 hasOpenAccess W2050081230 @default.
- W2050081230 hasPrimaryLocation W20500812301 @default.
- W2050081230 hasRelatedWork W158779233 @default.
- W2050081230 hasRelatedWork W2006994535 @default.
- W2050081230 hasRelatedWork W2039321593 @default.
- W2050081230 hasRelatedWork W2050081230 @default.
- W2050081230 hasRelatedWork W2062502531 @default.
- W2050081230 hasRelatedWork W2149603938 @default.
- W2050081230 hasRelatedWork W2416852591 @default.
- W2050081230 hasRelatedWork W2612797151 @default.
- W2050081230 hasRelatedWork W2613429086 @default.
- W2050081230 hasRelatedWork W4377939783 @default.
- W2050081230 hasVolume "271" @default.
- W2050081230 isParatext "false" @default.
- W2050081230 isRetracted "false" @default.
- W2050081230 magId "2050081230" @default.
- W2050081230 workType "article" @default.