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- W2050129690 abstract "The whey protein pattern of milk from animals affected by mastitic inflammation was resolved by two-dimensional gel electrophoresis (2D-PAGE) and compared to milk from unaffected cows. Inflammation caused the appearance of four spots aligned at a molecular weight level of 26 kDa and over a pH-region of 5.0 to 6.4. The spots excised from 2D gels were treated with chymotrypsin and the resulting peptides analyzed by MALDI-TOF mass spectrometry and RP-HPLC. All four spots yielded highly similar chymotryptic peptide mass fingerprints as well as chromatographic peak patterns. A database search could identify the four spots as isoforms of the bovine prostaglandin D synthase (PGD-S). In one of the isoforms a defined cysteine residue was shown to be oxidized to a sulfonic acid." @default.
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- W2050129690 date "2002-01-01" @default.
- W2050129690 modified "2023-09-27" @default.
- W2050129690 title "Lipocalin-type prostaglandin D synthase in milk: a new biomarker for bovine mastitis" @default.
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- W2050129690 doi "https://doi.org/10.1016/s0090-6980(01)00175-7" @default.
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