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• W2050168863 abstract "The increased number of therapeutic targets has led to a growing need for screening methods enabling possible inhibitor compound selection. Information for new therapeutic targets has been found mostly from sequencing of the human genome but this knowledge cannot be directly converted into clinically relevant drug molecules. After target identification, the multistep drug development process takes many years and hundreds of millions of dollars are spent without certainty of the outcome. The first and the most critical step in the drug development process is hit selection. The optimal high throughput screening method should provide the highest possible number of true positive hits for further studies and lead discovery. The result should be achieved with low material consumption in a rapid and automated process. Radioactive label based methods are sensitive, but due to the problems arising from the radioactivity, luminescence-based methods have become increasingly popular in screening. In this review, the time-resolved luminescence based quenching resonance energy transfer (QRET) technique is discussed for primary screening." @default.
• W2050168863 created "2016-06-24" @default.
• W2050168863 creator A5034828415 @default.
• W2050168863 creator A5039372012 @default.
• W2050168863 date "2015-12-01" @default.
• W2050168863 modified "2023-10-02" @default.
• W2050168863 title "Quenching resonance energy transfer (QRET): a single-label technique for inhibitor screening and interaction studies" @default.
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• W2050168863 doi "https://doi.org/10.1016/j.nbt.2015.02.007" @default.
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