FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2050171234> ?p ?o ?g. }

• W2050171234 endingPage "3652" @default.
• W2050171234 startingPage "3642" @default.
• W2050171234 abstract "In Streptococcus mutans, both competence and bacteriocin production are controlled by ComC and the ComED two-component signal transduction system. Recent studies of S. mutans suggested that purified ComE binds to two 11-bp direct repeats in the nlmC-comC promoter region, where ComE activates nlmC and represses comC. In this work, quantitative binding studies and DNase I footprinting analysis were performed to calculate the equilibrium dissociation constant and further characterize the binding site of ComE. We found that ComE protects sequences inclusive of both direct repeats, has an equilibrium dissociation constant in the nanomolar range, and binds to these two direct repeats cooperatively. Furthermore, similar direct repeats were found upstream of cslAB, comED, comX, ftf, vicRKX, gtfD, gtfB, gtfC, and gbpB. Quantitative binding studies were performed on each of these sequences and showed that only cslAB has a similar specificity and high affinity for ComE as that seen with the upstream region of comC. A mutational analysis of the binding sequences showed that ComE does not require both repeats to bind DNA with high affinity, suggesting that single site sequences in the genome may be targets for ComE-mediated regulation. Based on the mutational analysis and DNase I footprinting analysis, we propose a consensus ComE binding site, TCBTAAAYSGT." @default.
• W2050171234 created "2016-06-24" @default.
• W2050171234 creator A5013395521 @default.
• W2050171234 creator A5014704682 @default.
• W2050171234 creator A5031924369 @default.
• W2050171234 creator A5035013800 @default.
• W2050171234 creator A5061093782 @default.
• W2050171234 creator A5069150690 @default.
• W2050171234 creator A5069226523 @default.
• W2050171234 creator A5071807694 @default.
• W2050171234 creator A5074745851 @default.
• W2050171234 creator A5089064818 @default.
• W2050171234 date "2011-07-15" @default.
• W2050171234 modified "2023-09-29" @default.
• W2050171234 title "Characterization of DNA Binding Sites of the ComE Response Regulator from Streptococcus mutans" @default.
• W2050171234 cites W1516582684 @default.
• W2050171234 cites W1559358669 @default.
• W2050171234 cites W1573406654 @default.
• W2050171234 cites W169648825 @default.
• W2050171234 cites W1853614524 @default.
• W2050171234 cites W1883937874 @default.
• W2050171234 cites W1952814327 @default.
• W2050171234 cites W1968549820 @default.
• W2050171234 cites W1970258574 @default.
• W2050171234 cites W1987041144 @default.
• W2050171234 cites W1988990552 @default.
• W2050171234 cites W1995924937 @default.
• W2050171234 cites W2009331672 @default.
• W2050171234 cites W2013437656 @default.
• W2050171234 cites W2013505287 @default.
• W2050171234 cites W2036114576 @default.
• W2050171234 cites W2038874225 @default.
• W2050171234 cites W2047929024 @default.
• W2050171234 cites W2048205348 @default.
• W2050171234 cites W2072359409 @default.
• W2050171234 cites W2074726414 @default.
• W2050171234 cites W2093569734 @default.
• W2050171234 cites W2100122962 @default.
• W2050171234 cites W2107757630 @default.
• W2050171234 cites W2108141139 @default.
• W2050171234 cites W2108281648 @default.
• W2050171234 cites W2108559568 @default.
• W2050171234 cites W2111996478 @default.
• W2050171234 cites W2114837947 @default.
• W2050171234 cites W2123724054 @default.
• W2050171234 cites W2123974237 @default.
• W2050171234 cites W2124714829 @default.
• W2050171234 cites W2125375876 @default.
• W2050171234 cites W2127890671 @default.
• W2050171234 cites W2129585139 @default.
• W2050171234 cites W2130558864 @default.
• W2050171234 cites W2139222416 @default.
• W2050171234 cites W2140195816 @default.
• W2050171234 cites W2143109478 @default.
• W2050171234 cites W2147051160 @default.
• W2050171234 cites W2148576186 @default.
• W2050171234 cites W2151378885 @default.
• W2050171234 cites W2169589212 @default.
• W2050171234 doi "https://doi.org/10.1128/jb.00155-11" @default.
• W2050171234 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3133340" @default.
• W2050171234 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21602345" @default.
• W2050171234 hasPublicationYear "2011" @default.
• W2050171234 type Work @default.
• W2050171234 sameAs 2050171234 @default.
• W2050171234 citedByCount "42" @default.
• W2050171234 countsByYear W20501712342012 @default.
• W2050171234 countsByYear W20501712342013 @default.
• W2050171234 countsByYear W20501712342014 @default.
• W2050171234 countsByYear W20501712342015 @default.
• W2050171234 countsByYear W20501712342016 @default.
• W2050171234 countsByYear W20501712342017 @default.
• W2050171234 countsByYear W20501712342018 @default.
• W2050171234 countsByYear W20501712342019 @default.
• W2050171234 countsByYear W20501712342020 @default.
• W2050171234 countsByYear W20501712342021 @default.
• W2050171234 countsByYear W20501712342022 @default.
• W2050171234 crossrefType "journal-article" @default.
• W2050171234 hasAuthorship W2050171234A5013395521 @default.
• W2050171234 hasAuthorship W2050171234A5014704682 @default.
• W2050171234 hasAuthorship W2050171234A5031924369 @default.
• W2050171234 hasAuthorship W2050171234A5035013800 @default.
• W2050171234 hasAuthorship W2050171234A5061093782 @default.
• W2050171234 hasAuthorship W2050171234A5069150690 @default.
• W2050171234 hasAuthorship W2050171234A5069226523 @default.
• W2050171234 hasAuthorship W2050171234A5071807694 @default.
• W2050171234 hasAuthorship W2050171234A5074745851 @default.
• W2050171234 hasAuthorship W2050171234A5089064818 @default.
• W2050171234 hasBestOaLocation W20501712342 @default.
• W2050171234 hasConcept C104317684 @default.
• W2050171234 hasConcept C107824862 @default.
• W2050171234 hasConcept C141231307 @default.
• W2050171234 hasConcept C153911025 @default.
• W2050171234 hasConcept C22427896 @default.
• W2050171234 hasConcept C2778021871 @default.
• W2050171234 hasConcept C2778276568 @default.
• W2050171234 hasConcept C2778430296 @default.
• W2050171234 hasConcept C2779377703 @default.
• W2050171234 hasConcept C2780368718 @default.

• next