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• W2050237977 abstract "The metabolism of 2-acetylaminofluorene (AAF) to its six oxidative metabolites has been used to study cytochrome P450 monooxygenase activity in two rat hepatoma cell lines, McA-RH7777 and Reuber H4-II-E. McA-RH7777 cells exhibited considerably higher basal activities than H4-II-E cells for all metabolic pathways studied. Phenobarbital induced AAF metabolite formation in McA-RH7777 cells to a similar extent as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), but was only a weak inducer of these activities in H4-II-E cells. Northern blot analysis utilizing specific phenobarbital or 3-methylcholanthrene inducible cytochrome P450 cDNA probes indicated that there was at least a 10-fold increase in a 3-methylcholanthrene inducible cytochrome P450 transcript in phenobarbital treated McA-RH7777 cells. These data suggest that in this transformed cell line phenobarbital behaves as a polycyclic hydrocarbon-like inducer." @default.
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• W2050237977 date "1986-05-01" @default.
• W2050237977 modified "2023-09-26" @default.
• W2050237977 title "Induction by phenobarbital in McA-RH7777 rat hepatoma cells of a polycyclic hydrocarbon inducible cytochrome P450" @default.
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• W2050237977 doi "https://doi.org/10.1016/0006-291x(86)91184-8" @default.
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