Matches in SemOpenAlex for { <https://semopenalex.org/work/W2050357321> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2050357321 abstract "The adenyl cyclase of the oxyntic, or acid-secreting, cells of bullfrog gastric mucosa has been found to be a membrane-bound enzyme. A method has been developed to isolate the adenyl cyclase rich membrane fractions in a hypotonic medium containing dithiothreitol, which has been found to protect the hormonal resposivenes of the adenyl cyclase.Highest specific activity of adenyl cyclase was localized in a light membrane fraction which also had abundant K+-stimulated ATPase and K+-stimulated p-nitrophenyl phophatase and very low cytochrome c oxidase activty. The three gastric secretagogues tested, namely histamine, pentagastrin and methylcholine, significantly stimulated the adenyl cyclase activity of the light membrane fraction.After treatment with 10 mM Mg+ further subfractionation of the light membrane fraction on a sucrose density gradient yielded light membrane subfraction 1, light membrane subfraction 2 and light membrane subfraction 3 in order of increasing densities. The three subfractions had different enzymatic and chemical properties. Adenyl cyclase activity has been found to be distributed in all three subfractions. However, the hormonal responsiveness of the three fractions was quite different. Light membrane subfraction 2 could be stimulated by all three secretagogues, light membrane subfraction 1 by histamine and methylcholine, while light membrane subfraction 3 was refractory to all three secretagogues. On the basis of the cholesterol to phospholipid molar ratio, RNA content, glycoprotein content and the enzymatic data it is suggested that light membrane subfraction 1 and light membrane subfraction 2 are of general plasma-membrane type, while light membrane subfraction 3 is largely of cytoplasmic origin." @default.
- W2050357321 created "2016-06-24" @default.
- W2050357321 creator A5062720676 @default.
- W2050357321 creator A5078614839 @default.
- W2050357321 date "1974-09-01" @default.
- W2050357321 modified "2023-09-27" @default.
- W2050357321 title "Adenyl cyclase of oxyntic cells its association with different cellular membranes" @default.
- W2050357321 cites W11795403 @default.
- W2050357321 cites W131092108 @default.
- W2050357321 cites W1500454132 @default.
- W2050357321 cites W1513897103 @default.
- W2050357321 cites W1566099493 @default.
- W2050357321 cites W1591872616 @default.
- W2050357321 cites W1608839454 @default.
- W2050357321 cites W1775749144 @default.
- W2050357321 cites W1969043149 @default.
- W2050357321 cites W1979630757 @default.
- W2050357321 cites W1986472994 @default.
- W2050357321 cites W2002662130 @default.
- W2050357321 cites W2031468523 @default.
- W2050357321 cites W2037197303 @default.
- W2050357321 cites W2064459911 @default.
- W2050357321 cites W2073426292 @default.
- W2050357321 cites W2073879684 @default.
- W2050357321 cites W2074949244 @default.
- W2050357321 cites W2077283281 @default.
- W2050357321 cites W2077516938 @default.
- W2050357321 cites W2083577457 @default.
- W2050357321 cites W2085214862 @default.
- W2050357321 cites W2232470632 @default.
- W2050357321 cites W2394950875 @default.
- W2050357321 cites W2404740933 @default.
- W2050357321 cites W2415166149 @default.
- W2050357321 cites W2437765934 @default.
- W2050357321 cites W2472310996 @default.
- W2050357321 cites W4301905694 @default.
- W2050357321 doi "https://doi.org/10.1016/0005-2736(74)90072-8" @default.
- W2050357321 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4376693" @default.
- W2050357321 hasPublicationYear "1974" @default.
- W2050357321 type Work @default.
- W2050357321 sameAs 2050357321 @default.
- W2050357321 citedByCount "27" @default.
- W2050357321 crossrefType "journal-article" @default.
- W2050357321 hasAuthorship W2050357321A5062720676 @default.
- W2050357321 hasAuthorship W2050357321A5078614839 @default.
- W2050357321 hasConcept C1122143 @default.
- W2050357321 hasConcept C12554922 @default.
- W2050357321 hasConcept C134018914 @default.
- W2050357321 hasConcept C181199279 @default.
- W2050357321 hasConcept C185592680 @default.
- W2050357321 hasConcept C2778918659 @default.
- W2050357321 hasConcept C2780069284 @default.
- W2050357321 hasConcept C41625074 @default.
- W2050357321 hasConcept C55493867 @default.
- W2050357321 hasConcept C86803240 @default.
- W2050357321 hasConceptScore W2050357321C1122143 @default.
- W2050357321 hasConceptScore W2050357321C12554922 @default.
- W2050357321 hasConceptScore W2050357321C134018914 @default.
- W2050357321 hasConceptScore W2050357321C181199279 @default.
- W2050357321 hasConceptScore W2050357321C185592680 @default.
- W2050357321 hasConceptScore W2050357321C2778918659 @default.
- W2050357321 hasConceptScore W2050357321C2780069284 @default.
- W2050357321 hasConceptScore W2050357321C41625074 @default.
- W2050357321 hasConceptScore W2050357321C55493867 @default.
- W2050357321 hasConceptScore W2050357321C86803240 @default.
- W2050357321 hasLocation W20503573211 @default.
- W2050357321 hasLocation W20503573212 @default.
- W2050357321 hasOpenAccess W2050357321 @default.
- W2050357321 hasPrimaryLocation W20503573211 @default.
- W2050357321 hasRelatedWork W1518579998 @default.
- W2050357321 hasRelatedWork W1524053175 @default.
- W2050357321 hasRelatedWork W1562281848 @default.
- W2050357321 hasRelatedWork W1643374090 @default.
- W2050357321 hasRelatedWork W2023793283 @default.
- W2050357321 hasRelatedWork W2032161274 @default.
- W2050357321 hasRelatedWork W2039254145 @default.
- W2050357321 hasRelatedWork W2158007132 @default.
- W2050357321 hasRelatedWork W2169178224 @default.
- W2050357321 hasRelatedWork W3110055093 @default.
- W2050357321 isParatext "false" @default.
- W2050357321 isRetracted "false" @default.
- W2050357321 magId "2050357321" @default.
- W2050357321 workType "article" @default.