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• W2050359969 abstract "Extracts of surface proteins, with minimal artifacts from contaminating cytosolic components, are highly desirable for investigating surface factors involved in the attachment and formation of biofilms by bacteria that are problematic in commercial food processing facilities. In this study, we compared the protein profiles of the food pathogen, Listeria monocytogenes, recovered after applying different surface protein extraction methods compiled from the literature: trypsin-enzymatic shaving with BICAM/sucrose or Tris/sucrose buffers (Tryp B+S, Tryp T+S), Tris-buffered urea (UB), lithium chloride (LiCl) and Tris-buffered urea applied with hypotonic-stressed cells (UB-Ghost), and subjected them to liquid chromatography tandem mass spectrometry and protein identification. The data indicate that the UB-Ghost extraction method provides a cleaner extract of surface proteins including the predicted (this study and the literature) or validated members (literature) from L. monocytogenes. This was determined by an accumulative lower unique peptide number exhibited by mass spectrometry for total cytoplasmic proteins among different surface extracts, with a majority of proteins demonstrating hydrophilic properties. The extracted proteins were from different functional categories and have associations with the cell surface, intermediary metabolism, information pathways, or functionally unknown proteins as suggested by in silico analyses performed by other groups (Leger and ListiList). The utilization of an optimized method for surface protein extraction should greatly facilitate identification by LC-MS/MS that could be useful to anyone working on molecular proteomics of bacterial surfaces." @default.
• W2050359969 created "2016-06-24" @default.
• W2050359969 creator A5005721561 @default.
• W2050359969 creator A5049052642 @default.
• W2050359969 creator A5070661341 @default.
• W2050359969 date "2015-03-01" @default.
• W2050359969 modified "2023-09-29" @default.
• W2050359969 title "Comparison of five methods for direct extraction of surface proteins from Listeria monocytogenes for proteomic analysis by orbitrap mass spectrometry" @default.
• W2050359969 cites W1499713822 @default.
• W2050359969 cites W1510002394 @default.
• W2050359969 cites W1537641994 @default.
• W2050359969 cites W1550349849 @default.
• W2050359969 cites W1574151267 @default.
• W2050359969 cites W1582419748 @default.
• W2050359969 cites W1620630009 @default.
• W2050359969 cites W1783084949 @default.
• W2050359969 cites W180910982 @default.
• W2050359969 cites W18398869 @default.
• W2050359969 cites W1941913237 @default.
• W2050359969 cites W1965096019 @default.
• W2050359969 cites W1970786550 @default.
• W2050359969 cites W1975304761 @default.
• W2050359969 cites W1981320207 @default.
• W2050359969 cites W1993183798 @default.
• W2050359969 cites W1999689722 @default.
• W2050359969 cites W2006201402 @default.
• W2050359969 cites W2006958280 @default.
• W2050359969 cites W2006986579 @default.
• W2050359969 cites W2016309466 @default.
• W2050359969 cites W2022157647 @default.
• W2050359969 cites W2026701302 @default.
• W2050359969 cites W2028819914 @default.
• W2050359969 cites W2030931278 @default.
• W2050359969 cites W2031181661 @default.
• W2050359969 cites W2033776526 @default.
• W2050359969 cites W2050650050 @default.
• W2050359969 cites W2055212290 @default.
• W2050359969 cites W2068391177 @default.
• W2050359969 cites W2068826818 @default.
• W2050359969 cites W2075778843 @default.
• W2050359969 cites W2077890471 @default.
• W2050359969 cites W2079568089 @default.
• W2050359969 cites W2079602979 @default.
• W2050359969 cites W2087711149 @default.
• W2050359969 cites W2094070743 @default.
• W2050359969 cites W2094201044 @default.
• W2050359969 cites W2095144222 @default.
• W2050359969 cites W2098364431 @default.
• W2050359969 cites W2099317703 @default.
• W2050359969 cites W2100837269 @default.
• W2050359969 cites W2105913048 @default.
• W2050359969 cites W2107993914 @default.
• W2050359969 cites W2108632205 @default.
• W2050359969 cites W2110856081 @default.
• W2050359969 cites W2111119053 @default.
• W2050359969 cites W2111485203 @default.
• W2050359969 cites W2112078820 @default.
• W2050359969 cites W2115933717 @default.
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• W2050359969 cites W2121860020 @default.
• W2050359969 cites W2124874453 @default.
• W2050359969 cites W2126610667 @default.
• W2050359969 cites W2132674054 @default.
• W2050359969 cites W2133001143 @default.
• W2050359969 cites W2133567480 @default.
• W2050359969 cites W2139239302 @default.
• W2050359969 cites W2147203214 @default.
• W2050359969 cites W2148465086 @default.
• W2050359969 cites W2148805491 @default.
• W2050359969 cites W2152770371 @default.
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• W2050359969 cites W2167152465 @default.
• W2050359969 cites W2170141161 @default.
• W2050359969 cites W2171091522 @default.
• W2050359969 cites W2326562780 @default.
• W2050359969 cites W2766276685 @default.
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• W2050359969 doi "https://doi.org/10.1016/j.mimet.2015.01.004" @default.
• W2050359969 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25578509" @default.
• W2050359969 hasPublicationYear "2015" @default.
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