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• W2050363430 startingPage "4452" @default.
• W2050363430 abstract "We show that selective labeling of proteins with protonated amino acids embedded in a perdeuterated matrix, dubbed ‘proton clouds’, provides general access to long-range contacts between nonexchangeable side chain protons in proton-detected solid-state NMR, which is important to study protein tertiary structure. Proton-cloud labeling significantly improves spectral resolution by simultaneously reducing proton line width and spectral crowding despite a high local proton density in clouds. The approach is amenable to almost all canonical amino acids. Our method is demonstrated on ubiquitin and the β-barrel membrane protein BamA." @default.
• W2050363430 created "2016-06-24" @default.
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• W2050363430 date "2014-01-31" @default.
• W2050363430 modified "2023-10-04" @default.
• W2050363430 title "Proton Clouds to Measure Long-Range Contacts between Nonexchangeable Side Chain Protons in Solid-State NMR" @default.
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• W2050363430 doi "https://doi.org/10.1021/ja412870m" @default.
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