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- W2050430167 abstract "A method to determine proteolytic activity using radial diffusion in agar gel in combination with protein adsorbed to a polystyrene surface as substrate is developed. After the agar gel is removed, the effect of enzyme activity is demonstrated by condensation of water vapor on the protein-coated surface. Wettability of the enzyme-affected surface is decreased compared to that of the unaffected protein surface. The method is tested with two enzymes, trypsin and pronase. The sensitivity of the method is on a par with other highly sensitive methods. With bovine serum albumin (BSA) as substrate, the activity in 1 ng of enzyme is detected in a 10 μl test volume. A linear relationship is found between the enzyme-affected surface and the logarithmic value of the enzyme concentration from 0.1 μg/ml to 1 mg/ml. With 5 μg/ml BSA in distilled water, the polystyrene surface is sufficiently coated for use in the assay. The method also permits quantitative protease determination in crude and contaminated samples. This sensitive and simple technique has a high degree of accuracy and reproducibility without several of the disadvantages associated with other types of methods." @default.
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- W2050430167 date "1981-11-01" @default.
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- W2050430167 title "Determination of proteolytic activity: A sensitive and simple assay utilizing substrate adsorbed to a plastic surface and radial diffusion in gel" @default.
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- W2050430167 doi "https://doi.org/10.1016/0003-2697(81)90185-8" @default.
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