FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2050437092> ?p ?o ?g. }

• W2050437092 endingPage "93" @default.
• W2050437092 startingPage "85" @default.
• W2050437092 abstract "Hsp16.5, a small heat-shock protein (sHSP) from hyperthermophilic archaeon, forms a homogeneous complex comprised of 24 subunits with a molecular mass of 400 kDa. This complex self-organizes under physiological conditions, and the structure of the complex is a nanoscale spherical capsule with small pores. Furthermore, this natural nanocapsule exhibits very high thermal stability. In this paper, we functionalized the nanocapsule to control the structure in response to external stimuli such as a protease signal and temperature. For this purpose, several mutations (Mut1–10) to create a cleavage site for a specific protease, Factor Xa, were introduced on the outer surface of the nanocapsule using a genetic engineering strategy. The resulting mutants were expressed to high levels in Escherichia coli. One of these mutants, Mut6, which has the most accessible cleavage site located at the triangular pore on the surface of the capsule, formed a spherical assembly similar to that observed for the wild-type protein. Mut6 showed the highest sensitivity to Factor Xa, and the structure of the protease digested Mut6 disassembled irreversibly after heating. In contrast, the nanocapsule comprising the wild-type Hsp16.5 was not influenced by the dual stimuli. These results suggest that Mut6 acts as a stimulus-responsive nanocapsule. Such a characteristic of the protein-based nanocapsule has attractive potential as a versatile intelligent system." @default.
• W2050437092 created "2016-06-24" @default.
• W2050437092 creator A5008991478 @default.
• W2050437092 creator A5011849208 @default.
• W2050437092 creator A5012263872 @default.
• W2050437092 creator A5022655580 @default.
• W2050437092 creator A5024717041 @default.
• W2050437092 creator A5028699398 @default.
• W2050437092 creator A5053687082 @default.
• W2050437092 creator A5072295594 @default.
• W2050437092 date "2009-01-01" @default.
• W2050437092 modified "2023-10-05" @default.
• W2050437092 title "Molecular design of protein-based nanocapsules for stimulus-responsive characteristics" @default.
• W2050437092 cites W1790977108 @default.
• W2050437092 cites W1968048573 @default.
• W2050437092 cites W1969501683 @default.
• W2050437092 cites W1973246211 @default.
• W2050437092 cites W1983207417 @default.
• W2050437092 cites W1995886976 @default.
• W2050437092 cites W2004602103 @default.
• W2050437092 cites W2015642465 @default.
• W2050437092 cites W2034429504 @default.
• W2050437092 cites W2045033562 @default.
• W2050437092 cites W2049831929 @default.
• W2050437092 cites W2053377662 @default.
• W2050437092 cites W2058221324 @default.
• W2050437092 cites W2068419479 @default.
• W2050437092 cites W2070930138 @default.
• W2050437092 cites W2080150861 @default.
• W2050437092 cites W2111220039 @default.
• W2050437092 cites W2112518808 @default.
• W2050437092 cites W2114815411 @default.
• W2050437092 cites W2115608429 @default.
• W2050437092 cites W2122522178 @default.
• W2050437092 cites W2123253454 @default.
• W2050437092 cites W2123630792 @default.
• W2050437092 cites W2128235529 @default.
• W2050437092 cites W2138816938 @default.
• W2050437092 cites W2146275007 @default.
• W2050437092 cites W2147092181 @default.
• W2050437092 cites W2150635644 @default.
• W2050437092 cites W2158986880 @default.
• W2050437092 cites W2160061574 @default.
• W2050437092 cites W2167719547 @default.
• W2050437092 cites W2170743354 @default.
• W2050437092 cites W2738623976 @default.
• W2050437092 doi "https://doi.org/10.1016/j.bmc.2008.11.013" @default.
• W2050437092 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19041251" @default.
• W2050437092 hasPublicationYear "2009" @default.
• W2050437092 type Work @default.
• W2050437092 sameAs 2050437092 @default.
• W2050437092 citedByCount "21" @default.
• W2050437092 countsByYear W20504370922012 @default.
• W2050437092 countsByYear W20504370922013 @default.
• W2050437092 countsByYear W20504370922014 @default.
• W2050437092 countsByYear W20504370922015 @default.
• W2050437092 countsByYear W20504370922016 @default.
• W2050437092 countsByYear W20504370922018 @default.
• W2050437092 countsByYear W20504370922019 @default.
• W2050437092 countsByYear W20504370922021 @default.
• W2050437092 countsByYear W20504370922022 @default.
• W2050437092 crossrefType "journal-article" @default.
• W2050437092 hasAuthorship W2050437092A5008991478 @default.
• W2050437092 hasAuthorship W2050437092A5011849208 @default.
• W2050437092 hasAuthorship W2050437092A5012263872 @default.
• W2050437092 hasAuthorship W2050437092A5022655580 @default.
• W2050437092 hasAuthorship W2050437092A5024717041 @default.
• W2050437092 hasAuthorship W2050437092A5028699398 @default.
• W2050437092 hasAuthorship W2050437092A5053687082 @default.
• W2050437092 hasAuthorship W2050437092A5072295594 @default.
• W2050437092 hasConcept C104317684 @default.
• W2050437092 hasConcept C12554922 @default.
• W2050437092 hasConcept C143065580 @default.
• W2050437092 hasConcept C155672457 @default.
• W2050437092 hasConcept C159985019 @default.
• W2050437092 hasConcept C171250308 @default.
• W2050437092 hasConcept C175156509 @default.
• W2050437092 hasConcept C181199279 @default.
• W2050437092 hasConcept C185592680 @default.
• W2050437092 hasConcept C192562407 @default.
• W2050437092 hasConcept C204399865 @default.
• W2050437092 hasConcept C205260736 @default.
• W2050437092 hasConcept C2776714187 @default.
• W2050437092 hasConcept C43369102 @default.
• W2050437092 hasConcept C55493867 @default.
• W2050437092 hasConcept C86803240 @default.
• W2050437092 hasConceptScore W2050437092C104317684 @default.
• W2050437092 hasConceptScore W2050437092C12554922 @default.
• W2050437092 hasConceptScore W2050437092C143065580 @default.
• W2050437092 hasConceptScore W2050437092C155672457 @default.
• W2050437092 hasConceptScore W2050437092C159985019 @default.
• W2050437092 hasConceptScore W2050437092C171250308 @default.
• W2050437092 hasConceptScore W2050437092C175156509 @default.
• W2050437092 hasConceptScore W2050437092C181199279 @default.
• W2050437092 hasConceptScore W2050437092C185592680 @default.
• W2050437092 hasConceptScore W2050437092C192562407 @default.
• W2050437092 hasConceptScore W2050437092C204399865 @default.
• W2050437092 hasConceptScore W2050437092C205260736 @default.

• next