Matches in SemOpenAlex for { <https://semopenalex.org/work/W2050478613> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2050478613 endingPage "247" @default.
- W2050478613 startingPage "247" @default.
- W2050478613 abstract "Tissue-type plasminogen activator (tPA) is one of the components of the plasminogen-plasmin (PLG-PLA) system, better known as fibrinolytic system for its role in the blood clot lysis. It has been demonstrated recently that the activation of plasminogen into the protease plasmin during the sperm-oocyte interaction in the pig and cow decreases the percentages of penetration and increases monospermy (Mondéjar et al. 2012). However, in the mouse species, it was showed that PLG-PLA system enhances fertilization (Huarte et al. 1993). Expression of tPA has been described in rat oocytes (Bicsak et al. 1989) and cumulus cells (Ny et al. 1987; O’Connell et al. 1987), but no clear evidence about its expression in mouse, pig, and cow oocytes or cumulus cells is available. We hypothesised that differences in the effect of PLG-PLA system on fertilization results between the species mentioned above could be related to differences in tPA expression. The aim of this study was the detection of mRNA encoding tPA in oocytes and cumulus cells in mouse, pig, and cow by molecular analysis. Total RNA was obtained from oocytes and cumulus cells and cDNA was synthesised with an oligo-dT as primer. These cDNAs were used as template in RT-PCR amplifications using specific primers designed based on the GenBank sequence for Mus musculus, Sus scrofa, and Bos taurus tPA (NM_ 008872, NM_214054, NM_174146, respectively). The results of this study showed a different expression in the 3 studied species. In mouse, amplicon encoding tPA was detected in oocytes and cumulus cells. In cow and pig, tPA transcripts were obtained only in cumulus cells. The relation between the differences in the tPA expression pattern and the role of PLG-PLA system on fertilization remains to be investigated. This study was supported by MICINN (AGL2009-12512-C02-01-02)." @default.
- W2050478613 created "2016-06-24" @default.
- W2050478613 creator A5000313816 @default.
- W2050478613 creator A5005018809 @default.
- W2050478613 creator A5013333510 @default.
- W2050478613 creator A5044640233 @default.
- W2050478613 creator A5076912115 @default.
- W2050478613 date "2013-01-01" @default.
- W2050478613 modified "2023-09-26" @default.
- W2050478613 title "196 EXPRESSION OF TISSUE-TYPE PLASMINOGEN ACTIVATOR IN OOCYTES AND CUMULUS CELLS FROM MOUSE, PIG, AND COW" @default.
- W2050478613 doi "https://doi.org/10.1071/rdv25n1ab196" @default.
- W2050478613 hasPublicationYear "2013" @default.
- W2050478613 type Work @default.
- W2050478613 sameAs 2050478613 @default.
- W2050478613 citedByCount "0" @default.
- W2050478613 crossrefType "journal-article" @default.
- W2050478613 hasAuthorship W2050478613A5000313816 @default.
- W2050478613 hasAuthorship W2050478613A5005018809 @default.
- W2050478613 hasAuthorship W2050478613A5013333510 @default.
- W2050478613 hasAuthorship W2050478613A5044640233 @default.
- W2050478613 hasAuthorship W2050478613A5076912115 @default.
- W2050478613 hasConcept C104317684 @default.
- W2050478613 hasConcept C153911025 @default.
- W2050478613 hasConcept C16685009 @default.
- W2050478613 hasConcept C181199279 @default.
- W2050478613 hasConcept C187882448 @default.
- W2050478613 hasConcept C196843134 @default.
- W2050478613 hasConcept C2776572282 @default.
- W2050478613 hasConcept C2776690073 @default.
- W2050478613 hasConcept C2779679481 @default.
- W2050478613 hasConcept C2781071845 @default.
- W2050478613 hasConcept C49105822 @default.
- W2050478613 hasConcept C54355233 @default.
- W2050478613 hasConcept C55493867 @default.
- W2050478613 hasConcept C71924100 @default.
- W2050478613 hasConcept C8185291 @default.
- W2050478613 hasConcept C86803240 @default.
- W2050478613 hasConcept C95444343 @default.
- W2050478613 hasConceptScore W2050478613C104317684 @default.
- W2050478613 hasConceptScore W2050478613C153911025 @default.
- W2050478613 hasConceptScore W2050478613C16685009 @default.
- W2050478613 hasConceptScore W2050478613C181199279 @default.
- W2050478613 hasConceptScore W2050478613C187882448 @default.
- W2050478613 hasConceptScore W2050478613C196843134 @default.
- W2050478613 hasConceptScore W2050478613C2776572282 @default.
- W2050478613 hasConceptScore W2050478613C2776690073 @default.
- W2050478613 hasConceptScore W2050478613C2779679481 @default.
- W2050478613 hasConceptScore W2050478613C2781071845 @default.
- W2050478613 hasConceptScore W2050478613C49105822 @default.
- W2050478613 hasConceptScore W2050478613C54355233 @default.
- W2050478613 hasConceptScore W2050478613C55493867 @default.
- W2050478613 hasConceptScore W2050478613C71924100 @default.
- W2050478613 hasConceptScore W2050478613C8185291 @default.
- W2050478613 hasConceptScore W2050478613C86803240 @default.
- W2050478613 hasConceptScore W2050478613C95444343 @default.
- W2050478613 hasIssue "1" @default.
- W2050478613 hasLocation W20504786131 @default.
- W2050478613 hasOpenAccess W2050478613 @default.
- W2050478613 hasPrimaryLocation W20504786131 @default.
- W2050478613 hasRelatedWork W1573149695 @default.
- W2050478613 hasRelatedWork W1938324726 @default.
- W2050478613 hasRelatedWork W1963639456 @default.
- W2050478613 hasRelatedWork W1968642255 @default.
- W2050478613 hasRelatedWork W2039215959 @default.
- W2050478613 hasRelatedWork W2040771719 @default.
- W2050478613 hasRelatedWork W2557781837 @default.
- W2050478613 hasRelatedWork W2558965337 @default.
- W2050478613 hasRelatedWork W2559333808 @default.
- W2050478613 hasRelatedWork W2559536337 @default.
- W2050478613 hasRelatedWork W2732971076 @default.
- W2050478613 hasRelatedWork W2772135581 @default.
- W2050478613 hasRelatedWork W2772212848 @default.
- W2050478613 hasRelatedWork W2772390526 @default.
- W2050478613 hasRelatedWork W2774339711 @default.
- W2050478613 hasRelatedWork W2903092770 @default.
- W2050478613 hasRelatedWork W2903540334 @default.
- W2050478613 hasRelatedWork W2989608579 @default.
- W2050478613 hasRelatedWork W2991195168 @default.
- W2050478613 hasRelatedWork W3120153243 @default.
- W2050478613 hasVolume "25" @default.
- W2050478613 isParatext "false" @default.
- W2050478613 isRetracted "false" @default.
- W2050478613 magId "2050478613" @default.
- W2050478613 workType "article" @default.