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- W2050491861 abstract "Abstract An individual mRNA species was isolated directly from lysates of Physarum polycephalum by hybridization to immobilised complementary DNA. The solvent for lysis was a 6 M-guanidinium salt solution, and the hybridization reaction was carried out at 42°C in 4 M guanidinium salt/33% (v/v) formamide buffer. The mRNA species isolated by this procedure was active in cell-free protein synthesis and directed the synthesis of a polypeptide of Mr 25 500 ± 750." @default.
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- W2050491861 date "1983-05-02" @default.
- W2050491861 modified "2023-09-26" @default.
- W2050491861 title "A single-step procedure for the isolation of individual mRNA species from crude lysates ofPhysarum polycephalum" @default.
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- W2050491861 doi "https://doi.org/10.1016/0014-5793(83)80212-9" @default.
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