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- W2050503072 abstract "Previously, we reported that Malassezia furfur, causing systemic fungal infection, was taken up into human monocytic cell line, THP-1, in a concentration-dependent manner. This fact suggested that M. furfur could activate phagocytes, such as monocyte and polymorphonuclear leukocyte. Thus we examined cytokine mRNA expression from human monocytic and granulocytic cell line, THP-1 and HL-60, stimulated with M. furfur by using reverse transcriptase-polymerase chain reaction (RT-PCR) and ELISA. We chose IL-1alpha, IL-6, IL-8, IL-12 and TNF-alpha as primers for THP-1, and IL-1alpha, IL-6 and IL-8 for HL-60. M. furfur induced the expression of IL-8 mRNA from THP-1 and HL-60 following incubation for 3 h, and also induced IL-1alpha mRNA from HL-60, although this induction was weaker than that of IL-8 mRNA. Furthermore, opsonized M. furfur induced stronger expression of IL-8 mRNA in comparison with intact M. furfur. IL-8 production from THP-1 and HL-60 was enhanced in a concentration- and incubation time-dependent manner. These facts strongly suggested that M. furfur could activate phagocytes, and could induce inflammatory responses in systemic infection." @default.
- W2050503072 created "2016-06-24" @default.
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- W2050503072 date "2000-06-01" @default.
- W2050503072 modified "2023-09-25" @default.
- W2050503072 title "Enhancement of IL-8 production from human monocytic and granulocytic cell lines, THP-1 and HL-60, stimulated withMalassezia furfur" @default.
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- W2050503072 doi "https://doi.org/10.1111/j.1574-695x.2000.tb01471.x" @default.
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