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- W2050523712 abstract "Summary Germ-free as well as conventional rats were dosed by proteinase-deficient Lactococcus lactis Bu-2-60 as a potential recipient strain to monitor colonization and lateral gene transfer. Lactococcus lactis MG1820 (pLMP1), Lactococcus lactis MG1820 (pAMβ1), or Lactococcus lactis MG1820 (pLMP1, pAMβ1) were applied as potential donor strains in different experiments. Lactococcus lactis MG1820 (pLMP1) contained a genetically modified proteinase gene, whereas Lactococcus lactis MG1820 (pAMβ1) carried the highly conjugative broad host-range plasmid pAMβ1. Lactococcus lactis MG1820 (pLMP1, pAMβ1) was constructed as a potential donor for both plasmids. Faecal and intestinal samples were analysed for the presence of the initially introduced strains and for lateral gene transfer of the modified proteinase gene and/or the plasmid pAMβ1 by applying conventional antibiotic resistance based screening techniques, in situ colony hybridization with specific probes, and diagnostic polymerase chain reaction. The germ-free rats were readily colonized by all administered strains, whereas the conventional rats were colonized only transiently. Lateral transfer could not be observed for the modified proteinase gene. However, the plasmid pAMβ1 was transferred to the lactococcal recipient strain and in one case to a strain or close relative of Enterococcus faecalis of the indigenous intestinal flora of a conventional rat." @default.
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- W2050523712 date "1996-08-01" @default.
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- W2050523712 title "Monitoring of genetically modified Lactococcus lactis in gnotobiotic and conventional rats by using antibiotic resistance markers and specific probe or primer based methods" @default.
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- W2050523712 doi "https://doi.org/10.1016/s0723-2020(96)80046-4" @default.
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