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- W2050565209 abstract "We have demonstrated earlier that human cells contain nuclear protein interacting with conserved GC-rich sequence motifs of human Alu-family DNA repeats. One of these sequences is located in the region between elements A and B of bipartite RNA polymerase III promoter of Alu (AB-region). In this study we have used a DNase I footprinting assay with an Alu restriction subfragment covering AB-region, as well as a gel mobility shift assay with appropriate synthetic oligonucleotides to analyse in more detail the interaction of the protein with AB-region. We have also used antibodies raised against a zinc-finger peptide to examine the presence of a zinc-finger in the Alu-binding protein. The results indicate that AGG triplets may be important for high-affinity binding of the protein to DNA, and that the Alu-binding protein is a zinc-finger protein." @default.
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- W2050565209 date "1991-07-01" @default.
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- W2050565209 title "Binding specificity of human nuclear protein interacting with the Alu-family DNA repeats" @default.
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- W2050565209 doi "https://doi.org/10.1016/0006-291x(91)90152-w" @default.
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