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• W2050574321 endingPage "25136" @default.
• W2050574321 startingPage "25112" @default.
• W2050574321 abstract "The significance of G-quadruplexes and the helicases that resolve G4 structures in prokaryotes is poorly understood. The Mycobacterium tuberculosis genome is GC-rich and contains >10,000 sequences that have the potential to form G4 structures. In Escherichia coli, RecQ helicase unwinds G4 structures. However, RecQ is absent in M. tuberculosis, and the helicase that participates in G4 resolution in M. tuberculosis is obscure. Here, we show that M. tuberculosis DinG (MtDinG) exhibits high affinity for ssDNA and ssDNA translocation with a 5' → 3' polarity. Interestingly, MtDinG unwinds overhangs, flap structures, and forked duplexes but fails to unwind linear duplex DNA. Our data with DNase I footprinting provide mechanistic insights and suggest that MtDinG is a 5' → 3' polarity helicase. Notably, in contrast to E. coli DinG, MtDinG catalyzes unwinding of replication fork and Holliday junction structures. Strikingly, we find that MtDinG resolves intermolecular G4 structures. These data suggest that MtDinG is a multifunctional structure-specific helicase that unwinds model structures of DNA replication, repair, and recombination as well as G4 structures. We finally demonstrate that promoter sequences of M. tuberculosis PE_PGRS2, mce1R, and moeB1 genes contain G4 structures, implying that G4 structures may regulate gene expression in M. tuberculosis. We discuss these data and implicate targeting G4 structures and DinG helicase in M. tuberculosis could be a novel therapeutic strategy for culminating the infection with this pathogen." @default.
• W2050574321 created "2016-06-24" @default.
• W2050574321 creator A5014930324 @default.
• W2050574321 creator A5033132821 @default.
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• W2050574321 creator A5041421289 @default.
• W2050574321 creator A5049222210 @default.
• W2050574321 creator A5059953302 @default.
• W2050574321 date "2014-09-01" @default.
• W2050574321 modified "2023-10-03" @default.
• W2050574321 title "Mycobacterium tuberculosis DinG Is a Structure-specific Helicase That Unwinds G4 DNA" @default.
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• W2050574321 doi "https://doi.org/10.1074/jbc.m114.563569" @default.
• W2050574321 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4155677" @default.
• W2050574321 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25059658" @default.

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