Matches in SemOpenAlex for { <https://semopenalex.org/work/W2050593492> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2050593492 endingPage "511" @default.
- W2050593492 startingPage "501" @default.
- W2050593492 abstract "Studies with a pair of Neurospora mutants deficient in aspartate transcarbamylase (carbamoylphosphate: l -aspartate carbamoyltransferase, EC 2.1.3.2) showed the following: 1. 1. Aspartate transcarbamylase activity could be reconstituted by incubation in vitro of ribosomes from one mutant with soluble proteins from the other, or vice versa . This system will be referred to as the ribosomal complementation system. Ribosomes from the two mutants failed to complement each other in vitro . 2. 2. The ribosomal complementation system did not require 2-mercaptoethanol unlike the system for complementation between soluble proteins in vitro . The 2-mercaptoethanol requirement of the soluble system could not be circumvented by the addition of ribosomes from a third mutant, which is transcarbamylase deficient and complementation negative. 3. 3. The aspartate transcarbamylase generated by the ribosomal complementation system remained ribosome-bound after repeated washings. 4. 4. The thermostability of the aspartate transcarbamylase generated by the soluble system depended upon the input ratio of the two mutant extracts, while the thermostability of the product of the ribosomal system was independent of the input ratio and indistinguishable from that of an aspartate transcarbamylase obtained by complementation in vivo . These results are interpreted as tentative indications that complementation is intercistronic. 5. 5. The ribosomal system is suitable for the study of hybrids between wild and mutant enzymes." @default.
- W2050593492 created "2016-06-24" @default.
- W2050593492 creator A5015161065 @default.
- W2050593492 creator A5017966953 @default.
- W2050593492 creator A5042756245 @default.
- W2050593492 creator A5069328134 @default.
- W2050593492 date "1970-06-01" @default.
- W2050593492 modified "2023-09-26" @default.
- W2050593492 title "Complementation on ribosomes between aspartate transcarbamylase mutants of Neurospora" @default.
- W2050593492 cites W1537250685 @default.
- W2050593492 cites W1967900318 @default.
- W2050593492 cites W2032341323 @default.
- W2050593492 cites W2082020789 @default.
- W2050593492 cites W2150830720 @default.
- W2050593492 doi "https://doi.org/10.1016/0005-2787(70)90747-1" @default.
- W2050593492 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/5453528" @default.
- W2050593492 hasPublicationYear "1970" @default.
- W2050593492 type Work @default.
- W2050593492 sameAs 2050593492 @default.
- W2050593492 citedByCount "7" @default.
- W2050593492 crossrefType "journal-article" @default.
- W2050593492 hasAuthorship W2050593492A5015161065 @default.
- W2050593492 hasAuthorship W2050593492A5017966953 @default.
- W2050593492 hasAuthorship W2050593492A5042756245 @default.
- W2050593492 hasAuthorship W2050593492A5069328134 @default.
- W2050593492 hasConcept C104317684 @default.
- W2050593492 hasConcept C143065580 @default.
- W2050593492 hasConcept C153911025 @default.
- W2050593492 hasConcept C166342909 @default.
- W2050593492 hasConcept C181199279 @default.
- W2050593492 hasConcept C188082640 @default.
- W2050593492 hasConcept C197248121 @default.
- W2050593492 hasConcept C2776739539 @default.
- W2050593492 hasConcept C2776769606 @default.
- W2050593492 hasConcept C2781216036 @default.
- W2050593492 hasConcept C54355233 @default.
- W2050593492 hasConcept C55493867 @default.
- W2050593492 hasConcept C67705224 @default.
- W2050593492 hasConcept C86803240 @default.
- W2050593492 hasConcept C88478588 @default.
- W2050593492 hasConceptScore W2050593492C104317684 @default.
- W2050593492 hasConceptScore W2050593492C143065580 @default.
- W2050593492 hasConceptScore W2050593492C153911025 @default.
- W2050593492 hasConceptScore W2050593492C166342909 @default.
- W2050593492 hasConceptScore W2050593492C181199279 @default.
- W2050593492 hasConceptScore W2050593492C188082640 @default.
- W2050593492 hasConceptScore W2050593492C197248121 @default.
- W2050593492 hasConceptScore W2050593492C2776739539 @default.
- W2050593492 hasConceptScore W2050593492C2776769606 @default.
- W2050593492 hasConceptScore W2050593492C2781216036 @default.
- W2050593492 hasConceptScore W2050593492C54355233 @default.
- W2050593492 hasConceptScore W2050593492C55493867 @default.
- W2050593492 hasConceptScore W2050593492C67705224 @default.
- W2050593492 hasConceptScore W2050593492C86803240 @default.
- W2050593492 hasConceptScore W2050593492C88478588 @default.
- W2050593492 hasIssue "2" @default.
- W2050593492 hasLocation W20505934921 @default.
- W2050593492 hasLocation W20505934922 @default.
- W2050593492 hasOpenAccess W2050593492 @default.
- W2050593492 hasPrimaryLocation W20505934921 @default.
- W2050593492 hasRelatedWork W1510898207 @default.
- W2050593492 hasRelatedWork W1593918510 @default.
- W2050593492 hasRelatedWork W2023880472 @default.
- W2050593492 hasRelatedWork W2028175821 @default.
- W2050593492 hasRelatedWork W2050593492 @default.
- W2050593492 hasRelatedWork W2093043491 @default.
- W2050593492 hasRelatedWork W2114298584 @default.
- W2050593492 hasRelatedWork W2148485332 @default.
- W2050593492 hasRelatedWork W2152631654 @default.
- W2050593492 hasRelatedWork W243920189 @default.
- W2050593492 hasVolume "209" @default.
- W2050593492 isParatext "false" @default.
- W2050593492 isRetracted "false" @default.
- W2050593492 magId "2050593492" @default.
- W2050593492 workType "article" @default.