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- W2050841008 abstract "Mouse peritoneal macrophages whether harvested from a stimulated or unstimulated peritoneal cavity are mitotically quiescent. Either of these populations can be subdivided based upon the particular conditions needed to bring particular subsets back into the cell cycle. Three subsets of macrophages are evident in exudates obtained after stimulation in vivo with starch, endotoxin, or thioglycolate medium. About 50% of such cells proliferate in vitro in response to macrophage growth factor (MGF). For about one-third of MGF-responsive cells, a non-specific agent given in vitro is sufficient for recruitment into the cell cycle (subset 1). Non-specific agents given were dextrans, endotoxin, or thioglycolate. For the remaining two-thirds of MGF-responsive cells, it appears that a need for MGF must be fulfilled in vitro (subset 2). There is always a fraction of macrophages from stimulated peritonea that appear to be non-responsive (subset 3). Unstimulated animals yield two subsets of macrophages in culture: about 80–90% non-responsive cells (subset a) with the remaining fraction only responsive to MGF and a non-specific agent given in combination (subset b). It is possible that growth responsiveness may be used as a criterion to define macrophage activation at the single-cell level within populations that on the whole are activated or normal." @default.
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- W2050841008 date "1978-08-01" @default.
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- W2050841008 title "Necessary and sufficient conditions for recruitment of macrophages into the cell cycle" @default.
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- W2050841008 doi "https://doi.org/10.1016/0014-4827(78)90416-0" @default.
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