FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2050873218> ?p ?o ?g. }

• W2050873218 endingPage "81" @default.
• W2050873218 startingPage "69" @default.
• W2050873218 abstract "The mechanism of sarcoplasmic reticulum (SR) ATPase Mg2+-dependent phosphorylation from Pi was investigated in the presence of 15% v/v dimethyl sulfoxide at pH 6, 20 degrees C, and in the absence of potassium. Measurements of intrinsic fluorescence changes and of 32P-labeled phosphoprotein (*E-P) were in agreement, both at equilibrium and in transient situations. We found that the amount of phosphoenzyme present and its rate of formation depended solely on the concentration of the (Mg X Pi) complex. Up to 6 nmol of phosphate/mg of protein was covalently bound to the enzyme, implying almost complete phosphorylation. Oxygen exchange experiments were also performed in order to allow calculation of the absolute rate constant of *E-P hydrolysis to the noncovalent complex (0.8-1.0 s-1), which differs from the observed rate of enzyme dephosphorylation (0.3-0.5 s-1); in addition, they allowed calculation of the bimolecular rate constant of substrate binding (2-2.4 M-1 s-1). The results demonstrate that in the presence of dimethyl sulfoxide, phosphorylation occurs by the following simple mechanism: relatively slow binding of the neutral substrate (Mg X Pi), with poor affinity, followed by a thermodynamically favorable formation of the covalent bond between phosphate and the possibly hydrophobic active site. The interaction between magnesium and calcium-deprived SR vesicles was studied in the presence of 0-20% v/v dimethyl sulfoxide (or 0-30% v/v glycerol) at pH 7 and 20 degrees C. The presence of either solvent led to the disappearance of the two typical pH-dependent effects we previously characterized for magnesium: loss of the Mg2+-induced spectral shift of tryptophan fluorescence emission and loss of the biphasic pattern displayed by the intrinsic fluorescence rise after addition of calcium to Ca2+-deprived Mg2+-preincubated vesicles. In the absence of solvent, the interaction of magnesium with the calcium-deprived ATPase was also characterized from the point of view of phosphoenzyme formation from ATP or Pi at pH 7 in the absence of potassium: we found that calcium-independent phosphorylation was slower when phosphate was added to SR vesicles preincubated with magnesium that when magnesium was added to vesicles preincubated with phosphate, suggesting that preincubation with magnesium had depleted the phosphate-reactive conformation of the ATPase. A simple reaction scheme for phosphoenzyme formation is described: it implies that the (Mg X Pi) complex is a substrate for this reaction, whereas the Mg2+ itself acts as a pH-dependent, dimethyl sulfoxide sensitive inhibitor of full enzyme phosphorylation.(ABSTRACT TRUNCATED AT 400 WORDS)" @default.
• W2050873218 created "2016-06-24" @default.
• W2050873218 creator A5003389694 @default.
• W2050873218 creator A5037277718 @default.
• W2050873218 creator A5072718587 @default.
• W2050873218 creator A5089938597 @default.
• W2050873218 date "1985-01-01" @default.
• W2050873218 modified "2023-09-24" @default.
• W2050873218 title "Interaction of magnesium and inorganic phosphate with calcium-deprived sarcoplasmic reticulum adenosine triphosphatase as reflected by organic solvent induced perturbation" @default.
• W2050873218 cites W114387737 @default.
• W2050873218 cites W1492725068 @default.
• W2050873218 cites W1493463750 @default.
• W2050873218 cites W1495648604 @default.
• W2050873218 cites W1503904460 @default.
• W2050873218 cites W1507548413 @default.
• W2050873218 cites W1511723719 @default.
• W2050873218 cites W1520370615 @default.
• W2050873218 cites W1524916420 @default.
• W2050873218 cites W1547616639 @default.
• W2050873218 cites W1552252619 @default.
• W2050873218 cites W1555275400 @default.
• W2050873218 cites W1570126443 @default.
• W2050873218 cites W1577031595 @default.
• W2050873218 cites W1590933500 @default.
• W2050873218 cites W1594905039 @default.
• W2050873218 cites W1602420438 @default.
• W2050873218 cites W1623935262 @default.
• W2050873218 cites W1646738393 @default.
• W2050873218 cites W1965727628 @default.
• W2050873218 cites W1970174862 @default.
• W2050873218 cites W1971434319 @default.
• W2050873218 cites W1972895522 @default.
• W2050873218 cites W1973436202 @default.
• W2050873218 cites W1975815704 @default.
• W2050873218 cites W1979934156 @default.
• W2050873218 cites W1980759541 @default.
• W2050873218 cites W1982011214 @default.
• W2050873218 cites W1982108498 @default.
• W2050873218 cites W1982504490 @default.
• W2050873218 cites W1992498265 @default.
• W2050873218 cites W1999568159 @default.
• W2050873218 cites W2021861197 @default.
• W2050873218 cites W2029029518 @default.
• W2050873218 cites W2038028482 @default.
• W2050873218 cites W2039322515 @default.
• W2050873218 cites W2046320679 @default.
• W2050873218 cites W2060383918 @default.
• W2050873218 cites W2063404335 @default.
• W2050873218 cites W2079809851 @default.
• W2050873218 cites W2088839366 @default.
• W2050873218 cites W2118865044 @default.
• W2050873218 cites W2135393487 @default.
• W2050873218 cites W2222846765 @default.
• W2050873218 cites W330955888 @default.
• W2050873218 doi "https://doi.org/10.1021/bi00322a012" @default.
• W2050873218 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3158341" @default.
• W2050873218 hasPublicationYear "1985" @default.
• W2050873218 type Work @default.
• W2050873218 sameAs 2050873218 @default.
• W2050873218 citedByCount "137" @default.
• W2050873218 countsByYear W20508732182012 @default.
• W2050873218 countsByYear W20508732182013 @default.
• W2050873218 countsByYear W20508732182014 @default.
• W2050873218 countsByYear W20508732182015 @default.
• W2050873218 countsByYear W20508732182020 @default.
• W2050873218 crossrefType "journal-article" @default.
• W2050873218 hasAuthorship W2050873218A5003389694 @default.
• W2050873218 hasAuthorship W2050873218A5037277718 @default.
• W2050873218 hasAuthorship W2050873218A5072718587 @default.
• W2050873218 hasAuthorship W2050873218A5089938597 @default.
• W2050873218 hasConcept C11960822 @default.
• W2050873218 hasConcept C121332964 @default.
• W2050873218 hasConcept C130316041 @default.
• W2050873218 hasConcept C148898269 @default.
• W2050873218 hasConcept C178666793 @default.
• W2050873218 hasConcept C178790620 @default.
• W2050873218 hasConcept C179104552 @default.
• W2050873218 hasConcept C180577832 @default.
• W2050873218 hasConcept C181199279 @default.
• W2050873218 hasConcept C181912034 @default.
• W2050873218 hasConcept C185592680 @default.
• W2050873218 hasConcept C2777061661 @default.
• W2050873218 hasConcept C2777132085 @default.
• W2050873218 hasConcept C2778452553 @default.
• W2050873218 hasConcept C2780471494 @default.
• W2050873218 hasConcept C41625074 @default.
• W2050873218 hasConcept C519063684 @default.
• W2050873218 hasConcept C543218039 @default.
• W2050873218 hasConcept C55493867 @default.
• W2050873218 hasConcept C62520636 @default.
• W2050873218 hasConcept C71240020 @default.
• W2050873218 hasConcept C93391505 @default.
• W2050873218 hasConceptScore W2050873218C11960822 @default.
• W2050873218 hasConceptScore W2050873218C121332964 @default.
• W2050873218 hasConceptScore W2050873218C130316041 @default.
• W2050873218 hasConceptScore W2050873218C148898269 @default.
• W2050873218 hasConceptScore W2050873218C178666793 @default.
• W2050873218 hasConceptScore W2050873218C178790620 @default.

• next